Diego A. Peralta Resume - 2016

Diego A. Peralta Resume - 2016

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  Diego A. Peralta 2145Ransom Oaks Dr. Columbus Columbus, OH 43228 (614) 931-1856 [email protected] Education 05/14 Bachelor of Science in Microbiology magna cum laude distinction, University of South Florida Honors College – Tampa, FL  GPA: 3.7  Microbiology Student Excellence Award – Department of Cell Biology, Microbiology and Molecular Biology – 5/14  USF Research and Innovation Student Research Award - USF Honors College – 4/14  Undergraduate Student Award Pharmaceutical Science Neuroscience Research Outstanding Presentation – USF Health – 2/14  Dean’s List of Scholars – USF College of Arts and Sciences – 12/11 Relevant Courses: General Chemistry w/lab (I, II), Organic Chemistry w/lab (I, II), Biology w/lab (I, II), Physics w/lab (I, II), Biochemistry, Genetics, Cell Biology w/lab, Cell Metabolism, General Microbiology w/lab, Virology, Determinative Bacteriology w/lab, Molecular Microbiology, *Seminars in Microbiology Laboratory Skills Biochemical Laboratory experience with: Cell Culture and Cell Lines, Drug Screening and Discovery, In-vitro and In-vivo Drug Treatment, InCell Western, Western Blotting, Biochemical Fractionation, Immunostaining and Immunofluorescence Imaging, Behavioral and Biochemical assays and their optimization, C. elegans strains maintenance and growth and culture media preparation; Light/Fluorescence Microscopy, Restriction Enzyme Mapping of Plasmid DNA, Subcellular Fractionation, Harvesting, DNA/RNA/Protein Isolation and Concentration Determination, Titration, Chemical Compound Synthesis and Isolation, UV/Mass Spectroscopy Microbiology Lab experience with: Culture Media Preparation, Bacterial Staining, Pure Culture Isolation, Bacterial Viable Quantification, Bacteriophages and Plaque Assay, Fungal Slide Culture, Antimicrobial Susceptibility Testing, GasPak Anaerobic System Bacteriology Identification by:  Media/Test: TSA, TSI, Gelatin, Casein, Starch, Tween 80, Catalase, MSA, BAP, MacConkey, EMB, SS, TSI, Citrate, BEA, Saline NaCl, Coagulase, Bile Solubility/SDS, Oxidase, CTA Sugars, CAMP, Optochin Sensitivity, Bacitracin Differentiation, Nitrate Reduction, Mueller Hinton, Henrici, Martin Lewis
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  (chocolate agar), XLD,HEA, Nutrient Enriched, Brucella, Indole, Methyl Red, Voges-Proskauer, Phenol Red Glucose, Urease, Phenylalanine, Decarboxylation, Malonate, API 20 E Strip, Co-agglutination (Lim Group B Strep, Phadebact Strep B), Slide Agglutination, RPR Card  Staining: Gram, Differential, Dorner, Malachite Green, Acid-Fast/Ziehl-Neelsen, Nigrosin, Congo Red, Capsule, Flagella  Motility: Semi-Solid Agar, Simple Mount, Hanging Drop Publications/Patents 08/15  “An optimized InCell Western screening technique identifies hexachlorophene as a novel potent TDP43 targeting drug” Published on Journal of Biotechnology Volume 2017, 10 August 2015; Second Author 06/15  “Method of Treating TDP-43 Proteinopathies Using Hexachlorophene” U.S. Utility Patent Application filed June 17, 2015 Work Experience 08/15 – 05/16 Team Leader - Traveling Missionary NET Ministries, Inc - Saint Paul, MN  Planned event schedules and delegated the various components  Developed and delivered large group presentations  Lead small group discussions  Completed various administrative duties as assigned  Participated in regular evaluations by supervisory staff  Stayed with and interacted with hosts from different states and cities  Developed a daily schedule for the team  Assigned and delegated administrative and event responsibilities, and assisted team members in fulfilling assigned duties  Communicated with representatives from each location to be ministered to  Served as a weekly contact between the team and the NET home office  Assisted team members in developing skills necessary to minister  Ensured team members followed NET guidelines, policies, and direction given from the NET home office  Assisted team members in relating positively and effectively with each other  Collaborated with soup kitchens to feed and cloth indigent people  Worked with Emmy award-winning Spirit Juice Studios on a recruiting video production for NET Ministries to be released Fall 2016 08/14 – 05/15 Science Teacher Somerset Academy Charter Middle/High School - Pembroke Pines, FL  Provided students with the opportunity to explore the concepts of biological and physical sciences, and their applications to the everyday life that surrounds us  Classes taught included regular, advanced, gifted and pre-ap coursework
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   Curriculum included:Nature of Science, Weathering, Erosion, Deposition, and Landforms, Energy in the Earth System, Weather and Climate, Energy, Motion, and Forces, The Cell, Human Body Systems, Immunity, Disease, and Disorders, Earth’s Structures, Earth’s History, Human Impact on Earth, Waves and Light, Energy and Heat, Life over Time, Reproduction and Heredity, DNA and Modern Genetics, and Ecology  Instruction of the sciences included working with and specially planning and designing instruction to meet the unique needs of the following children: - Exceptional student education (ESE) students with a disability progress in school because of a physical, mental, emotional, social or learning exceptionality - English-language learners (ELL) students who were unable to communicate fluently or learn effectively in English, who often came from non-English- speaking homes and backgrounds  Prepared monthly consults for ESE and ELL students’ growth and development plan for their academic and social environment  Tutored students who lacked support for schooling at home  Held meetings for parents to inform them about the class curriculum as well as expectations for both students and parents  Maintained on going contact with all student parents and their child’s performance in the classroom as well as parent-teacher conferences when deemed necessary  Proctored state mandated tests  Participated in District and School Initiatives and promoted positive interactions with colleagues, students and parents  Mentored three out of four students in all of middle school for their science fair that won 1st, 2nd, and honorable mention in the Somerset Academy Science Fair. Further mentored one these students whom returned with a 4th place in the Broward County Regional Science and Engineering Fair  Applied and enforced student discipline in accordance with Student Code of Conduct and student handbook 10/14 – 02/15 Head Coach - Soccer Somerset Academy Charter Middle/High School - Pembroke Pines, FL  Turned program around by converting seven of last year’s losses to four wins and three ties, and by also scoring +17 more goals than the previous season  Implemented team mentality, sportsmanship and use of communicational skills for athletes  Provided athletes with proper nutrition guidance and training for the prevention of injuries  Instructed athletes with the skills necessary and correct technique for playing the sport  Evaluated and selected athletes during tryouts  Managed roster of 25 athletes for their: - Verification of student physical examination requirements
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  - Preparation ofathletic permission slips and eligibility lists - Athletic fees  Worked with the Athletic Director and Principal for the following: - Communicating of soccer schedule - Arrangement and communication of travel for away games - Correlate the practice schedules of JV & Varsity squads  Supervised practice fields and playing fields during and following periods of team participation  Remained with students and supervised them while traveling to away competitions  Cared for athletes and injured athletes  Cared for, maintained and oversaw the storage of all equipment and uniforms  Prepared award lists and season records for end of the year sports banquet  Communicated with players about personal development during the season as well as the off-season  Applied and enforced student discipline during athletic contests, practice sessions, and while on trips off school property in accordance with Student Code of Conduct and student handbook 9/10 – 7/12 Tutor Americorps/USF Patel Elementary - Tampa, FL  Tutored targeted students in reading and math  Maintained accurate and current records of student contact and service  Worked in collaboration with school staff to monitor student progress  Appraised student progress using evaluation instruments  Served as a mentor and positive role model to targeted students Experiences/Volunteering 09/12 – 06/14 ResearchAssistant USF Health Johnnie B. Byrd, Sr. Alzheimer's Center & Research Institute - Tampa, FL  Included in the Focus on Research Newsletter – March, 2014  University of South Florida Undergraduate Student Award Winner in Pharmaceutical Science Neuroscience Research – Feb, 2014  Characterized a TDP-43 protein targeting drug by using C. elegans based ALS model  Screened and discovered novel drugs for the characterization, treatment and regulation of lab generated in-vitro and in-vivo models of neurodegenerative diseases  Laboratory work included but was not limited to Cell Culture and Cell Lines, Drug Screening and Discovery, In-vitro and In-vivo Drug Treatment, InCell Western, Western Blotting, Biochemical Fractionation, Immunostaining and Immunofluorescence Imaging, Behavioral and Biochemical assays and their optimization, C. elegans strains maintenance and growth and culture media preparation
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  12/12 – 07/14 SurgeryWaiting Room Front Desk Patient Care Management and Preoperative Services Moffitt Cancer Center and Research Institute, Tampa, FL *Service Recognition Award Recipient  Provided comfort and support to patients’ family and friends by serving as a liaison between Pre-Op Holding, Inpatient Recovery and Outpatient Recovery  Greeted and registered family members/patient’s friends in the waiting room  Escorted patients and families/friends to the Pre-Op Area , Recovery Room and Special Care Unit  Transferred incoming telephone calls for patients/family members to the surgery waiting room  Checked the coffee service area to make sure there were ample supplies and re-stocked supplies; maintained neat area and ensured magazines and coloring books were stocked  Offered assistance, directions, and comfort as needed; made sure families/friends in the waiting room were comfortable and had their questions answered  Contacted resource nurse, Patient Relations or Moffitt Chaplain and escorted families/friends experiencing extreme distress into a consultation room  Ensured confidentiality by appropriately handling the surgical schedule  Presented a cheerful and helpful presence at all times to families/friends that were stressed and did not act in their customary manner; provided comfort and support while understanding and respecting privacy  Opened and closed the surgery waiting area  Handled computer operations for reference to families/friends of patient surgery status  Served as a translator and communicator between staff and Spanish- speaking-only patients, family and friends 12/12 – 07/14 Infusion Center Department Assistant Moffitt Cancer Center and Research Institute, Tampa, FL *Service Recognition Award Recipient  Supported patients who were receiving chemotherapy and assisted the Infusion Staff  Checked for correct patient identification and escorted patients from the waiting room area  Ensured patient was comfortable and offered blankets, pillows and refreshments  Ran errands, picked up supplies, delivered and kept supplies stocked and organized  Visited with the patients or family members to help pass time  Maintained order in the kitchens by making sure that coffee, cups, etc. were
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  always available forpatients and their visitors  Placed lunch orders by 9:30 AM for patients on infusion (including BMT infusion)  Escorted patients to infusion chairs for treatment or to next appointment if needed  Unplugged patient’s IV pump upon request to use the restroom and escorted patients to washroom  Maintained cleanliness of waiting area and coffee service station  Ordered all supplies for lobby area  Escorted new patients and families upon request with attached script information  Served as a translator and communicator between staff and Spanish- speaking-only patients, family and friends 12/12 – 07/14 Computed Tomography Department Assistant Moffitt Cancer Center and Research Institute, Tampa, FL *Service Recognition Award Recipient  Improved the efficiency of the radiation therapy service by assisting patients who were waiting for treatment and also contributed to physical and/or emotional comfort of patients  Was available in the treatment area and at the request of any Radiation Therapist, called and escorted the Therapist patient to his/her treatment room from the patient waiting area  Offered physical assistance the patient needed if the patient was not being attended by hospital staff  Offered conversation or assistance for patients and/or guests waiting outside treatment rooms  Escorted the patient out of the treatment area if hospital staff was not attending the patient and he/she appeared confused or physically unsteady or unable to leave the area  Updated the Radiation Therapists patient schedule and logged in the number and information of patients escorted on the transport STAT sheet in the Radiology Handbook  Served as a translator and communicator between staff and Spanish- speaking-only patients, family and friends 02/13 - 02/14 Post Anesthesia Care Unit Assistant Shriners Hospitals for Children, Tampa, FL *Service Recognition Award Recipient  Assisted the staff in the PACU Department while they were prepping patients and assisted patients as they woke from surgery  Assisted with making beds and setting up prep area for patients  Stocked bedside cabinets with patient care items  Escorted family to the PACU as the patient woke up from surgery  Transported patients to other departments of the Hospital when needed  Assembled patient chart packets and organize files
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   Assisted withpushing beds and carts  Served as a liaison between the nurses (PACU) and the patient’s family  Oriented families to surroundings during the patient visits in the PACU  Answered telephone and provided information to the staff  Helped with errands related to patients and family members  Provided assistance with offering magazines, newspapers to the patients in the PACU and to waiting families and friends  Assisted with maintaining a neat and professional environment in the waiting area  Served as a translator and communicator between staff and Spanish- speaking-only patients, family and friends 11/13 – 05/14 Pre-Med Physician Observational Program Sarcoma Department Moffitt Cancer Center and Research Institute, Tampa, FL  Shadowed orthopedic oncology surgeon Dr. David Cheong and medical oncologist Dr. Damon Reed during surgeries and their day-to- day interactions with patients and guests 01/14 – 04/14 Presenter - *Seminars in Microbiology USF Department of Cell Biology, Microbiology and Molecular Biology - Tampa, FL  Presented personal research and peer reviewed research papers to undergraduate and graduate level student classrooms, professors and PhD judges Certifications  Laboratory Biosafety  Science Laboratory Safety  Educator's Certificate in General Science grades 5th through 9th  Content area teacher for English speakers of other languages  Child Abuse and Neglect  VIRTUS protecting all children from child sexual abuse  Disability Awareness  Cardiopulmonary Resuscitation and First Aid  Disaster Preparedness and Response Additional Qualifications  Bilingual: can fully fluently speak and write in both English and Spanish  Experience working with Microsoft Office (Word, Excel, Powerpoint) and Microsoft Outlook, Quickbooks, Pinnacle, Chem Bio Draw Ultra, Drop Box, Copy, Good Notes, Notes Plus, and Audacity  Knowledge of internet, networking, and e-mail systems Abstracts Published in ResearchSymposiums 10/12 – 06/14
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  1. *Diego A.Peralta, Malathi Narayan, Enzo A. Nicosia, Ekta Sood, Redjon Zhuleku, Umesh K. Jinwal (2014) Screening and Identification of a Novel Drug Targeting TDP-43 for the Treatment of ALS. 24th Annual USF Health Research Day, Tampa, FL, February 21. *Best presentation award 2. *Malathi Narayan, Juan Zhang, Enzo A. Nicosia, Ekta Sood, Kaitlyn Braswell, Diego A. Peralta, Redjon Zhuleku, Daniel C. Lee, Sheeba Varghese-Gupta, Umesh K. Jinwal (2014) Withaferin A regulates Parkinson’s Disease-associated protein LRRK2. 24th Annual USF Health Research Day, Tampa, FL, February 21.*Best presentation award 3. Redjon Zhuleku, Kaitlyn Braswell, Malathi Narayan, Enzo A. Nicosia, Diego A. Peralta, Ekta Sood, Umesh K. Jinwal (2014) Tau & α-Synuclein expressing Caenorhabiditis elegans: Generation and Characterization for Neurodegenerative Diseases. 24th Annual USF Health Research Day, Tampa, FL, February 21 4. Diego A. Peralta, Juan Zhang, Kaitlyn Braswell, Umesh K. Jinwal (2013) Optimization of a novel InCell-Western technique for the screening of TDP- 43 targeting drugs. Undergraduate Research and Arts Colloquium, Florida, April 17th 5. Kaitlyn Braswell, Juan Zhang, Devki Patel, Twisha Jani, Maxx Ramirez, Diego Peralta, Daniel Lee, Umesh K. Jinwal (2013) New Drug Screen for the Reduction of Alpha-Synuclein. 1st Education and Research Forum 2013, Florida, March 27th 6. Kaitlyn Braswell, Juan Zhang, Devki Patel, Twisha Jani, Maxx Ramirez, Diego Peralta, Daniel Lee, and Umesh K. Jinwal (2013) Screening of Drugs for the Treatment of Synucleinopathies. The 23rd Annual USF Health Research Day, Florida, February 22nd