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differentialleucocytecount.pptx
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- 2. Smear prepration. Types of WBC and their counts Applied aspects. To draw a peripheral blood smear, stain it with Leishman's stain and do the differential count( DLC) of the given blood sample.
- 3. Crucial inthe body defense against pathogens Capable of phagocytosis. These are complete cells, with a nucleus and organelles They lack Hb so they are colorless (i.e. white) Able to move into and out of blood vessels • Can respond to chemicals released by damaged tissues.
- 5. •Granules in theircytoplasm can be stained inflammatory and allergic reactions. • Neutrophils, Eosinophil, and Basophils
- 6. Agranulocytes Lack visible cytoplasmicgranules Lymphocytes and Monocyte
- 7. Normal levels=4,000 to 11,000 cells/mm3 Abnormal leukocyte levels Leucocytosis -Above 11,000 leukocytes/ml Generally indicates an infection / Leukemia • Leucopenia - low leukocyte level Commonly caused by certain drug
- 13. Differential leukocyte countis performed on stained blood film. Material required Glass slides Blood sample Leishman's stain Buffer water (pH = 6.8) Cedar wood oil Microscope with oil immersion objective
- 14. Place asmall drop of blood on one end of a clean slide, in the middle. Hold a second slide (i.e., spreader) above the first, right angle of 30 to 450. Move the spreader forward rapidly, thus spreading the blood over the slide in a thin film. Leave the slide to air dry. A properly made film should dry quickly; if the film is not dried rapidly there will be rouleaux formation of RBC and the cells will appear shrunken. A good blood film should not cover the entire surface of the slide. It should be even, smooth and should be having three parts i.e., head, body and tail. Dried film should be stained with Leishman's stain.
- 15. Pour theLeishman's stain over the film to cover it completely. Keep it for two minutes. After one minute, buffer water is added gently to the stain already on the blood film to cover the slide completely (it is approximately double the volume of stain). Allow the diluted stain to act for about 8 to 10 minutes. Wash the film in buffer water or distilled water. The film should be rose pink in colour. Allow the film dry and focus under low power of microscope. Then put a drop of cedar wood oil and see under oil immersion objective. Count the WBCs in a zig-zag fashion across the breadth of film, in the body and tail junction area of film, until 100 leukocytes are counted. To record them draw a large square and divide it into 100 small squares. Identify one cell and write in each square. Count different WBCs coming across. The result thus obtained is directly in percentage.
- 19. N L RESULT: Polymorph % = Lymphocyte% = Eosinophil % = Monocyte % = Basophil % =