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Chromatography

This document discusses various chromatography techniques including paper chromatography, thin layer chromatography, partition chromatography, adsorption chromatography, ion-exchange chromatography, gel filtration chromatography, affinity chromatography, high performance liquid chromatography, and gas chromatography. The different techniques separate molecules based on properties like size, charge, and affinity. They have various applications in biochemistry like purifying proteins, antibodies, and separating biological compounds.

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CHROMATOGRAPHY Biochemistry of Medics 1
Chromatography Biochemistry of Medics 2
Chromatography Biochemistry of Medics 3
Chromatography- Principle Biochemistry of Medics 4
Chromatography- Classification Biochemistry of Medics 5
Chromatography- Classification(Contd.) Biochemistry of Medics 6
Partition Chromatography Biochemistry of Medics 7
Paper chromatography Biochemistry of Medics 8
Paper chromatography Biochemistry of Medics 9
Paper chromatography- Procedure Biochemistry of Medics 10
Paper chromatography- Procedure Biochemistry of Medics 11
Ascending and Descending Paper Chromatography Biochemistry of Medics 12
Paper Chromatography -Analysis Biochemistry of Medics 13
Rƒ value Biochemistry of Medics 14
Two dimensional chromatography Biochemistry of Medics 15
Significance of Paper chromatography Biochemistry of Medics 16
Thin layer chromatography (TLC) Biochemistry of Medics 17
TLC- Procedure Biochemistry of Medics 18
TLC -Overview Biochemistry of Medics 19
Significance of TLC Biochemistry of Medics 20
Advantages of TLC over paper chromatography Biochemistry of Medics 21
2)Adsorption chromatography Biochemistry of Medics 22
2)Adsorption chromatography- Elution Biochemistry of Medics 23
Adsorption chromatography The substance loosely adsorbed to the stationary medium comes in the earlier fraction. Biochemistry of Medics 24
Ion – Exchange chromatography Biochemistry of Medics 25
Ion – Exchange chromatography Biochemistry of Medics 26
Ion – Exchange chromatography- Procedure Biochemistry of Medics 27
Ion – Exchange chromatography- Procedure (Contd.) Biochemistry of Medics 28
Types of ion exchanges resins Biochemistry of Medics 29
Ion – Exchange chromatography- An Overview Biochemistry of Medics 30
4) Gel filtration chromatography or Molecular Sieve chromatography Biochemistry of Medics 31
Gel filtration chromatography or Molecular Sieve chromatography Biochemistry of Medics 32
Gel filtration chromatography-An Overview Larger particles come out first, while smaller particles come in later fractions Biochemistry of Medics 33
5) Affinity chromatography Biochemistry of Medics 34
Affinity chromatography- Significance Biochemistry of Medics 35
Affinity chromatography- Significance By using antibodies, antigens can be easily separated. Conversely, antibodies can be purified by passing through a column containing the antigen. Specific Antibody binds to a specific antigen attached to the stationary medium, the remaining antibodies come out in earlier fractions. Biochemistry of Medics 36
6) High performance liquid chromatography- Biochemistry of Medics 37
High performance liquid chromatography- Biochemistry of Medics 38
High performance liquid chromatography- Significance Biochemistry of Medics 39
High performance liquid chromatography- Apparatus Biochemistry of Medics 40
6) Gas liquid chromatography- Biochemistry of Medics 41
Gas liquid chromatography- Biochemistry of Medics 42
Gas liquid chromatography-Apparatus Biochemistry of Medics 43
Summary Biochemistry of Medics 44

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In this document
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An overview of chromatography introduced in the presentation as a fundamental technique in biochemistry.

Discussion of the principles that underlie chromatography and classifications of various types.

Focus on partition chromatography and its basic principles and applications in biochemistry.

Detailed procedures for performing paper chromatography, highlighting key steps involved.

Discussion on ascending and descending paper chromatography and methods for analysis.

Introduction to the Rƒ value, a critical metric for analyzing chromatography results.

Description of two-dimensional chromatography as a complex technique for separation.

Explains the importance and applications of paper chromatography in scientific research.

Overview, procedures, and significance of Thin Layer Chromatography in biochemistry.

Comparison of TLC and paper chromatography, noting the advantages of TLC as a method.

Details on adsorption chromatography, including elution procedures and key principles.

In-depth coverage of ion-exchange chromatography, its procedures, and types of resins used.

Explains gel filtration chromatography, its principle, and fragment behavior during separation.

Description and significance of affinity chromatography in biochemical separations using antibodies.

Overview of HPLC, its significance, and the apparatus used for conducting HPLC.

Introduction to gas liquid chromatography, its setup, and apparatus requirements.

A concluding summary of various chromatography techniques discussed in the presentation.

Chromatography