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Fishery smkn 1 suboh situbondo Jawa Timur Indonesia
The document provides instructions for inducing breeding of the catfish (Clarias gariepinus) using the hipofisation technique. This involves extracting hormones from the hypothalamus and pituitary glands of donor fish and injecting them into recipient broodstock to stimulate spawning. The steps described include preparing donor and recipient fish, extracting hormones, injecting fish, stripping eggs and sperm, mixing gametes, and spreading the eggs into a hatchery. Proper management of water quality, feeding of larvae, and counting hatching rates are also summarized. The goal is to intensify seed production through induced spawning outside of natural breeding cycles.
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Fishery smkn 1 suboh situbondo Jawa Timur Indonesia
- 1. GERMINATING OF THE CATFISH (Clariasgariepinus) USING INDUCE BREEDING by : fishery SMKN 1 SUBOH SITUBONDO – EAST JAVA
- 2. Breeding of Clariasgariepinus Background Hipofisation Donor Fish Recipient Fish Fasting Material and THE MATERIALS Instruments of Hipofisation Taking of Hipofisation Making of Hipofisation Liquid Broodstock Injection Striping of Female Broodstock Ovum Surgical Operation of Male Broodstock Sperm Mixing of Ovum and Sperm Spreading Into Hatchery Counting of The Hatchery Rate
- 3. • THE BACKGROUND Fish is one of important source protein for human being Inhibiting factor: lack of quality germ Causal factor: fish usually breeds naturally Applying of aquaculture biotechnology: induce breeding technique, using the method of hipofisation technique Back Next
- 4. HIPOFISATION TECNIQUE 1. isthe stimulated spawning using the extract of hipofisa gland. 2. Purpose : 3. - to stimulate spawning of the fish , 4. - to intensify the seeds production 5. to crossbreed by using induced fertilizing Back Next
- 5. PREPARING A DONOR FISH •CRITERIA ♠ From the same species, or fr om univer sal donor fish (Example : gold fish) ♠ Sexually Mature ♠ Prefer ably from the male br ood ♠ Healthy and disease fr ee Back Next ♠ T he weight is known
- 6. PREPARING THE RECIPIENT FISH SexuallyMature From Aquaculture Fish (Not wild fish) Healthy and normal (having no physical defect) The age meets the requirement/category Not A discarded Fish Having superior features/characteristic The weight is known Back Next
- 7. DIFFERENTIATING BETWEEN SEX OFCATFISH BROODSTOCK Male : in its sexual organ there is a protrusion pointed as the penis of male catfish Female : near its anus, there is a small protrusion marked by a reddish hole as its sexual organ of female catfish Back Next
- 8.
- 9. FEMALE BROODSTOCK MATURITY ISSIGNED BY The share of sex organ is spherical reddish, the hole is getting bigger Its movement is slower than before The colour of skin is brighter than before The stomach feels soft when touched Back Next
- 10. MALE BROODSTOCK MATURE ISSIGNED BY ITS SEXUAL ORGAN LOOKS POINTED AND REDDISH THE STOMACH IS SLENDER THE SKIN COLOUR IS DARKER THAN BEFORE Back Next
- 11. FASTING STAGE MEANING :FASTING THE FISH FOR A SEVERAL HOURS BEFORE BREEDING PURPOSE : • - REDUCING FAT AND THE FISH FECES THAT CAN DECREASE THE WATER QUALITY Back Next
- 12. THE MATERIALS • DONOR FISH AQUABIDEST ALCOHOL CLEARWATER COTTON / TISSUE FILTER PAPER SOLUTION OF 0,9% NaCl (Physiological Salt) Back Next
- 13. Tools TISSUE GRINDER CENTRIFUGE PINSET COTTON MANCHETE SPUITAND SYRINGE FISH CUSHION FILTER PAPER SCALE Back Next
- 14. MEASURING THE DOSAGE Recipientfemale 1 kg, dosage 1,5 ____meaning it needs the donor fish as weight as 1,5 kg Recipient male 0, 5 kg , dosage 1____ meaning it needs the donor fish as weight as 0,5 kg Back Next
- 15. TAKING THE HIPOFISA GLAND Thehead is cut Split from under the nose till separate Taking the brain carefully using a pinset Taking the hipofisa gland carefully using the pinset Cleaning with filter paper Back Next
- 16. Back Next The headis cut
- 17. gambar Back Next Split fromunder the nose till separate
- 18.
- 19. gambar Back Next Taking thehipofisa gland
- 20. EXTRACTING THE HIPOFISA GLAND Hipofisagland is attached to the tissue grinder and blended until tender or done Add 2 cc of aquabidest Sett the hipofisa using centrifuge for 2 minutes Let the hipofisa gland be in the reaction tube for ±1minute Taking the clear upper layer using a spuit Back Next
- 21.
- 22. gambar Back Next Add 2cc of aquabidest
- 23. gambar Back Next Dissolve thehypofisa gland using the spuit
- 24. gambar Back Next ut itin the reaction tube and centrifuge
- 25. gambar Back Next Let thehypofisa gland
- 26. Back Next Take theclear layer
- 27. Back Next The wayof injecting
- 28. DETERMINING THE DOSAGEOF OVAPRIM HORMON • The ovaprim hormon dosage is : • 0,5 ml/kg brood and diluted with 1 ml of aquadest
- 29. The male catfishbrood is put then operate it
- 30.
- 31. Slice it intopieces
- 32. Back Next Prepare thefemale catfish egg cell
- 33. Mixing the eggand sperm of broodstock Pour the sperm little by little into a bowl which is filled with the eggs, then stir slowly using chicken feather that has been sterilized Stir until all of the eggs mixed perfectly Wash off the eggs using aquadest Then spread it into hatchery Back Next
- 34. Back Next Mix theegg cell with the sperm
- 35. Back Next Wash itusing mineral water
- 36. Spreading the fishegg Preparing a hatchery tank Fill with fresh water and the height is 30 cm Aeration for several hours before, to increase the dissolved oxygen Drop adequate methylene blue to avoid the fungus attack If the temperature in the media is enough, the eggs are ready to spread Back Next
- 37. Back Next Spred intothe hatching container
- 38. Counting of fecunditycatfish eggs • Gravimetric methode • This method is also called as the weight method. • Fecundity = (The egg total weight : The egg sample weight) x The amount of eggs (in sample) • F = Fecundity • W = The egg total weight • w = The egg sample weight • N = The amount of eggs (in sample) •
- 39. Management of waterquality in the hatchery of catfish egg Because the egg that does not hatch decays faster , it causes the water quality decreases, so the water in hatchery must be circulated continuously After most of the eggs have hatched, the hatchery is cleaned from the eggs that do not hatch Technical requirement: ph 7-8, water temperature 28-29oC Back Next
- 40. Keeping the larvaof Clarias sp The egg will hatch in 24 hours For one day old, larva of clarias sp. needn’t feeding because it still has its own food For 4 day old, give it an egg yolk or Tubifex For 7 day old give it natural food as artemia and tubifex Maintain the water quality by continuous circulation Back Next
- 41. LARVA FEEDING the naturalfood usually given is artemia or tubifex The artificial food : powder food 200# or from the egg yolk Back Next
- 42. Counting the hatchingrate • Sampling method • Take the homogen larva in sampling way using a measurement glass • Total of larva= Total of sampling egg x the volume of water in the measurement glass • The rate of hatching = total of larva x 100% • fecundity • Back Next