Download to read offline
More Related Content
Similar to Formulation Of Poly Herbal Ointment all final (2).pptx
More from Rakesh Barik
Formulation Of Poly Herbal Ointment all final (2).pptx
- 1. Formulation Of Poly Herbal Ointment AndEvaluation Of Its Antimicrobial Activity
- 2.
- 3. POLY HERBAL OINTMENT PolyHerbal Ointment Is A Type Of Topical Medication Made From A Combination Of Several Herbs Or Plant Extracts. It Is Used To Treat A Variety Of Skin Conditions Such As Eczema, Psoriasis, Rashes, And Fungal Infections. The Combination Of Herbs Used In The Ointment Is Chosen Based On Their Therapeutic Properties, Which May Include Anti-inflammatory, Anti-bacterial, Anti-fungal, And Anti-viral Effects. Poly Herbal Ointments Can Be Formulated As Creams, Gels, Or Balms And Are Usually Applied Directly To The Affected Area Of The Skin. They Are Often Preferred Over Synthetic Medications Due To Their Natural Origin, Minimal Side Effects, And Potential For Synergistic Effects Among The Herbs Used.
- 4. Piper Betle Betel PlantsAre Cultivated For Their Leaves Which Are the Most Commonly Used As Flavoring In Chewing. Commonly Found In Malaysia,Vietnam, Cambodia, Thailand, And Myanmar. Chemical Constituents Present Chavibetol ,Safrole Eugenol, Isoeugenol USES 1. Helps In Wound Healing 2. Improves Oral Health 3. Anti Bacterial 4. Anti Inflammatory Kingdom:Plantae Division: Magnoliophyta Class: Magnoliopsida Order: Piperales Family: Piperaceae Genus: Piper Species: P. Betle 4
- 5. Tagetes Erecta There AreThree Main Types Of Marigolds: French, African, And Signet. African Marigolds Can Reach 3-4 Feet. French Marigolds (Tagetes Patula) Are Usually Between 6-12 Inches Tall. Signet Marigolds (Tagetes tenuifolia) Are The Smallest, Usually 6 Inches Or Less. Kingdom : Plantae Division : Magnoliophyta Class :Magnoliopsida Order :Asrerales Family :Calenduleae Genus :Calendula Uses Beneficial In Treating Ulcers, Piles, Kidney Problems, And Muscle Pain. Heals Skin Wounds, Burns, And Rashes. Insect repellent
- 6. Carica Papaya The PapayaIs A Small, Simply Branched Tree, Usually With A Single Stem Growing From 5 To 10 M. Papaya Skin, Pulp, And Seeds Contain A Variety Of Phytochemicals, Including Carotenoids And Polyphenols. Kingdom: Plantae Phylum: Tracheophyta Class: Magnoliopsida Order: Brassicales Family: Caricaceae Species: Carica Papaya Uses It Is Used To Reducing Inflammation, Improving Blood Sugar Control, Supporting Skin And Hair Health, And Preventing Cancer.
- 7. Ixora coccinea (Also KnownAs Jungle Geranium). Commonly Found In Southern India, Bangladesh And Sri Lanka. The Flowers, Leaves, Roots, And The Stem Are Used To Treat Various Ailments In The Indian Traditional System Of Medicine. Chemical Constituents Present Ursolic Acid, Oleanolic Acid, Sitosterol. Kingdom: Plantae Phylum: Magnoliophyta Class: Magnoliopsida Subclass: Asteridae Order: Gentianales Family: Rubiaceae Genus: Ixora Subject: Ixora coccinea L 7 Uses Antioxidative, Antibacterial, Gastroprotective, Hepatoprotective, Antidiarrhoeal, Antinociceptive, Antimutagenic, Antineoplastic, And Chemopreventive Effects. Used In Ayurveda
- 8. The Most WidelyGrown Species Of Annona And A Native Of Tropical Climate In The Americas West Indies, And In India. Chemical Constituents presents tannins, alkaloids, phenols, glycosides, flavonoids and steroids Pharmacological Properties And Biological Activities, Such As Antioxidant, Antimicrobial, Antidiabetic, Antiviral, Anticancer, And Hepatoprotective Activities. Kingdom: Plantae phylum: Tracheophyta Class : magnoliopsida Order: Magnoliales Family: Annonaceae Genes : Annona Species: Annona reticulate L 8 Annona Reticulata Uses The Plant Is Traditionally Used For The Treatment Of Epilepsy, Dysentery, Cardiac Problems, Worm Infestation, Constipation, Hemorrhage, Antibacterial Infection, Dysuria, Fever, Ulcer
- 9. 9 Collection Extraction Formulation of ointment Antibacterialactivity Materials And Methods
- 10. COLLECTION First We CollectedSome Herbal Leaves. Then We Shade Dried For 12 To 14 Days Before Being Ground Into Course Powdered It Was Kept In A Well Sealed Container Away From Climatic Changes Until Use. 10
- 11. Extraction Extraction involves removingthe ingredients from the raw medication by using solvents to separate them from it. Powered leaves was extracted by Decoction method. After Drying the Dried powder was taken into a beaker and add Petroleum Ether in it and then Kept For Heating For 1 Hour Then The Sample Was Filtered And The filtrate Was Collected And with the remaining Residue add 100ml of water heat it for 1hr,continue for 4to 5 times until the filtrate become colourless. Transfer the herbal extract into china dish evaporate it for until it gets semisolid consistency 11
- 12. Formulation of Ointment S.No Name of the ingredient Quantity to be taken 1 Cetostearyl alcohol 15 gms 2 PEG 20ml 12 S. No Name of the ingredient Quantity to be taken 1 Annona reticulata 0.8 gms 2 Carica Papaya 1 gms 3 Ixora coccinea 1 gms 4 Piperbetle 0.76 gms 5 Tagetes erecta 0.95 gms Table 1: Formulation of ointment base Table 2: Formulation of Herbal ointment Early Preparation of The Ointment Base Involved Precisely Weighing Cetostearyl Alcohol and PEG , Which Were Then Put In a Beaker On a Hot Plate. Then The Desired Amounts Of Herbal Extracts Were Added To This . Continue The Stirring Until It Gets Homogeneous Mixture And Then Transfer In To A Suitable Container The Fusion Method Is Used To Prepare The Ointment
- 13. Influence 13 EVALUATION OF ANTIBACTERIAL ACTIVITY Anti-microbialActivity Is The Practice Of Eradicating Or Preventing The Development Of Microorganisms. Antimicrobial Agents Either Kill Bacteria (Bactericidal) Or Prevent The Development Of Bacteria (Bacteriostatic). Pour Plate Method Preparation Of The Media Of The Media Preparation Of The Sample . Take nutrient agar in a conical flask add distilled water to it. . Close the conical flask with cotton ball. . Then autoclave it for 15min at 121˚c. Take 1 gm of prepared polyherbal ointment in a 9ml of solvent and dissolve it (serial dilution) . Take 1gm of marketed product (cetrimide)& in a dissolve in a 9ml of methanol. . Preparation of blank
- 14. INOCULATION Using A sterilemicropipette or calibrated pipette, place 1 ml of the diluted sample in the middle of the Petri plate. Flame the bottle's mouth by removing the lid. 15 ml of the sterilized, molten medium at the proper temperature should be poured over the sample. Then add the pseudomonas aeruginosa After securing the plate's cover, gently spin the plate to properly combine the sample with the medium. Often, A "S" or "8" form is swirled into the plate. . Place the plate in an inverted position and incubate it under the proper incubation conditions (typically 24 hours at 37°C). 14
- 15. 15 RESULT INTERPRETATION OFPOUR PLATE METHOD Colonies that have formed after the incubation are seen. There will be one viable microbial cell or one colony-forming unit per colony. If every colony belongs to the same kind, we can infer that there was only one type of microbial genus present in the sample. It's possible that various genera or species produce colonies of a similar kind. Count the colonies and calculate CFU/mL by using the formula This will provide the total number of living microorganisms in the provided sample. Dilutions Ointment Standard (Neosporin) Blank 101 3.5×103 2.9×103 5.5×103 102 3.8×104 2.3×104 6×104 103 3.4×105 1.5×105 4.9×105 104 2.7×106 1.3×106 3.5×106 105 1.4×107 1×107 2.6×107 106 1×108 0.5×108 1.8×108
- 16. 16 Disk Diffusion Method oOn The Culture Medium, The Bacterial Culture Was Added, And A Well Was Bored Through The Centre Of The Agar. After That, Various Samples And 0.05ml Standard Solutions Were Added To These Wells, And The Plates Were Then Incubated At 37°C Overnight For Observation. Inhibition Was Seen And Contrasted With The Control. By Examining The Zone Of Inhibition Surrounding Each Well, The Test Organism's Sensitivity To The Plant Extract Under Test Was Identified. Different Samples Zone diameter in mm(pseudomonas aeruginosa) Ointment 13.2±2.3 Standard 11.67±0.23 Blank 21.50±0.71
- 17.
- 18. 18 Extracts Peak ValuesFunctional Groups Piper Betle 3390 2931 1600 1384 1191 1122 Oh C-h C=o Ch2 C-o C-o Tagetes Erecta 3308 2942 1772 1599 1420 1080 C-o C-h C=o C=c Ch2 C-o Carica Papaya 3412 1614 1390 1117 O-h, N-h C=o Ch2 C-o Ixora coccinea 3374 2934 1608 1384 1121 O-h C-h C=o Ch2 C-o Annona Reticulata 3306 2927 1604 1419 1076 O-h C-h C=o Ch2 C-o
- 19. 19 FTIR spectrum ofwater extract of Ixora coccinea FTIR spectrum of water extract of Tagetes Erecta FTIR spectrum of water extract of Annona Reticulata FTIR spectrum of water extract of Carica Papaya FTIR spectrum of water extract of Piper Betle
- 20.
- 21.