Gel document system

Gel document system

• 1.
  Prasented By Gutti.Pavan Msc-I Biochemistry The Instituteof Science 4/9/2018 1Gel Document system
• 2.
  Gel Document System •Gel documentation , also known as Gel doc. system , Gel image system , or Gel imager. • It is widely used in molecular biology laboratories for imaging and documentation of nucleic acid and protein polyacrylamide or agarose gels typically stained with ethidium bromide . • Generally , a gel doc. is composed of ultraviolet ( UV ) light transilluminator , a hood to shield external light sources and CCTV camera for image capturing 4/9/2018 2Gel Document system
• 3.
  How Does EthidiumBromide stain DNA 4/9/2018 3Gel Document system
• 4.
  PRINCIPLE • Principle offluorescence with fluorescent staining of nucleic acids , a fluorescent substance that has bound to nucleic acid is exited by ultraviolet irradiation and emits fluorescent light. • Ethidium bromide binds specifically to nucleic acid and the amount of bonding depends on the molecular weight and concentration of the nucleic acid. • In other words , a band for a large amount will shine brighter ; conversely , fluorescence will be weaker for a band for a small amount. 4/9/2018 4Gel Document system
• 5.
  PURPOSE AND ANALYSIS •Photography of stained gel. • Print out of photographic data. • Image data is displayed in real time. • Image still displayed can be printed out with a video printer or saved to a compact flash media for future use 4/9/2018 5Gel Document system
• 6.
  COMPONENTS OF GELDOC. SYSTEM 4/9/2018 6Gel Document system
• 7.
  Gel Document SystemInstrumentation 4/9/2018 7Gel Document system
• 8.
  COMPONENTS OF GELDOC. SYSTEM Camera : Ultraviolet camera ranging from 1.4m upto 8.3m pixel. Lenses : All lanses are computer controlled and motor driven. Here lances automatically tracks upwards or downwards movements of sample using auto focus. Filters : There is an extensive range of emission filters use for an array of applications.  Over head illuminator : White light , Ultra violet light , and LED lighting option Visible light : For extending the transmitted light application. Visible light convert screen for visible light application. 4/9/2018 8Gel Document system
• 9.
  COMPONENTS OF GELDOC. SYSTEM Adjustable stage : This enables samples to be moved closer to the camera. Darkroom : dark room is suitable for advanced chemiluminescence, fluorescence and visible light application. An electronic auto-door lock with security function prevents interruption to long exposures. Transilluminator : It has a variable intensity setting and has a safety cut-off. 4/9/2018 9Gel Document system
• 10.
  QUANTITY ONE FROMBIORED  It is user friendly version windows based option.  It has a various tool for image capturing and transformation.  There is a separate tool for identifying , detecting and drawing lense and bands.  There is a separate function for storing standard data , standard curve for known molecular weight and size.  A separate volume analysis tool for volume contour , free hand , rectangle circle and array.  The software also has options for VNTR display , VNTR calculation , differential display and colony counting applictions. 4/9/2018 10Gel Document system
• 11.
  Gel Document SystemApplications  1-D Electrophoresis Documentation  2-D Documentation  Protein Gel Documentation  Blotting technique analysis  Colony Counting  Post Translational Modifications 4/9/2018 11Gel Document system
• 12.
  REFERENCE  https://www.biocompare.com/Editorial-Articles/122215-Gel-Documentation- Systems  https://www.researchgate.net/topic/Gel-Documentation-System https://www.americanlaboratory.com/914-Application-Notes/1374-21st- Century-Gel-Documentation-in-Research-and-Production-Environments  https://www.the-scientist.com/?articles.view/articleNo/13610/title/Exposing- Gel-Documentation-Systems  https://www.the-scientist.com/?articles.view/articleNo/18733/title/Picture- Perfect  http://www.obrnutafaza.hr/pdf/witeg/Gel-Documentation.pdf  https://www.biocompare.com/Editorial-Articles/41637-Gel-Documentation- Systems 4/9/2018 12Gel Document system
• 13.
  4/9/2018 13Gel Documentsystem