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GREEN SYNTHESIS AND BIOLOGICAL EVALUATION OF CuO AND CeO NANOPARTICLES FROM PSIDIUM GUAJAVA.pptx
- 2. GREEN SYNTHESIS ANDBIOLOGICAL EVALUATION OF CuO AND CeO NANOPARTICLES FROM PSIDIUM GUAJAVA This Photo by Unknown author is licensed under CC BY-NC-ND.
- 3. Contents Introduction Material and methods Results Conclusion FutureProspects This Photo by Unknown author is licensed under CC BY-NC-ND.
- 4. Green Synthesis iseco- friendly Cost effective method Low Toxicity
- 5. Cerium and copper oxides Nanoparticels Nanoparticleshave more chemical reactivity as their bulk counterparts due to their surface-to-volume ratio quantum size effect Copper and Cerium exhibit antimacrobial and antibacterial activity
- 6. PSIDIUM GUAJAVA • PsidiumGuajava traditionally used as medical plant. The most bioactive compound in guava is quercetin (flavonoide). • Antibacterial • Accessibility • Anticancer
- 7. Objectives of the study •The present study aimed at green synthesis of cerium oxide and copper oxide nanoparticles using Psidium guajava leave using co- precipitation method optimized by response surface methodology. • It also studied the antibacterial effect of the synthesized cerium and copper nanoparticles. • This research helpful in offering a significant, cost effective and environmentally friendly synthesis approach for nanostructured CuO.
- 8. Materials and methods PREPARATIONOF EXTRACT FABRICATION OF NANOPARTICLES CHARACTERIZATION BIOLOGICAL EVALUATION
- 9. Preparation of theExtract
- 10. Optimization of independent parameters byresponse surface methodology for copper oxide nanoparticels Factor 1 Factor 2 Factor 3 Std Run A:Extarct Amount B:Salt Amount C:Sittiring Time ml ml Min. 19 1 55 55 225 20 2 55 55 225 4 3 100 100 30 1 4 10 10 30 15 5 55 55 225 13 6 55 55 -102.95 6 7 100 10 420 7 8 10 100 420 3 9 10 100 30 10 10 130.681 55 225 17 11 55 55 225 2 12 100 10 30 8 13 100 100 420 18 14 55 55 225 5 15 10 10 420 11 16 55 -20.6807 225 9 17 -20.6807 55 225 14 18 55 55 552.95 16 19 55 55 225 12 20 55 130.681 225
- 11. Optimization of independent parameters byresponse surface methodology for cerium oxide nanoparticels FACTOR 1 FACTOR 2 FACTOR 3 Run Psidium Guajava extract CeCl3.7H2O (0.5 M) Stirring time ml ml min 1 110 110 30 2 62.5 62.5 -126.812 3 142.385 62.5 260 4 62.5 62.5 646.812 5 62.5 142.385 260 6 62.5 62.5 260 7 62.5 62.5 260 8 15 15 30 9 62.5 -17.3852 260 10 110 15 490 11 -17.3852 62.5 260 12 110 110 490 13 62.5 62.5 260 14 15 110 30 15 62.5 62.5 260 16 15 110 490 17 62.5 62.5 260 18 62.5 62.5 260 19 110 15 30 20 15 15 490
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- 14. Results and Discussion • UVanalysis
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- 17. Effect of theindependent variables on the response copper oxide nanoparaticles
- 18. Effect of theindependent variables on the response cerium oxide nanoparaticles
- 19. Antibacterial activity Zone ofinhibition (mm) Concentrations (µg/mL) Escherichia coli (Gram-negative) Bacillus subtilus (Gram-positive) Copper oxide nanoparticles 20 14 ±0.10 16±0.04 40 17±0.09 19±0.06 60 20±0.05 21±0.07 80 24±0.04 25±0.02 Cerium oxide nanoparticles 20 11±0.12 14±0.04 40 13±0.9 16±0.05 60 16±0.07 19±0.09 80 19±0.03 22±0.06 Psidium gujava extract 20 9±0.04 13±0.04 40 12±0.05 15±0.05 60 14±0.08 18±0.06 80 17±0.07 21±0.09 Ciprofloxacin (standard) 20 10±0.03 14±0.03 40 9.5±0.03 15±0.04 60 12±0.06 17±0.06 80 13±0.05 18±0.07
- 20. CONCLUSION • In conclusion,our research on the green synthesis of copper oxide (CuO) and cerium oxide (CeO2) nanoparticles from Psidium guajava has yielded promising results. These nanoparticles exhibit remarkable antimicrobial efficacy
- 21. Future Prospects To maximizemetal nanoparticels impact, future studies should delve into • advanced characterization • biocompatibility assessment • while also focusing on scaling up production and fostering collaboration with industry partners.
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