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Similar to hemolytic anemia powerpoint.pptx general medicine
hemolytic anemia powerpoint.pptx general medicine
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- 2. • Hemolytic anemiais defined as anemia due to a shortened survival of circulating red blood cells (RBCs) due to their premature destruction. • It shares the following features • 1. A shortened red cell life span below the normal 120 days • 2.Elevated erythropoietin level and compensatory increase in erythropoiesis • 3.Accumulation of hemoglobin degradation products that are created as a part of process of red cell hemolysis
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- 5. LAB EVALUATION OFHEMOLYSIS
- 6. CLASSIFICATION OF HEMOLYTICANEMIAS THE COURSE OF THE DISEASE ACUTE CHRONIC THE PLACE OF RBC DESTRUCTION INTRAVASCULAR EXTRAVASCULAR THE WHENCE ACQUIRED IINHERITED
- 7. INTRAVASCULAR VS EXTRAVASCULAR Intravascular •Red cells lyse in the circulation and release their products into the plasma fraction. • Anemia • Decreased Haptoglobin • Hemoglobinemia • Hemoglobinuria • Urine hemosiderin • Increased LDH
- 8. Extravascular • ingestion ofred cells by macrophages in the liver, spleen and bone marrow • Little or no hemoglobin escapes into the circulation • Anemia • Decreased Haptoglobin • Normal plasma hemoglobin
- 9. CLINICAL FEATURES Clinical signand symptoms of hemolytic anemia depend upon the severity as well as duration of hemolysis. These are • Pallor • Jaundice • Splenomegaly • Gall stones • Skeletal abnormalities in severe hemolysis • Leg ulcers • Dyspnoea • Tachycardia and systolic murmur
- 10. DIAGNOSIS • 1. Historyof the patient • 2. Peripheral blood film • 3. Bone marrow findings • 4. Biochemical tests • 5. Other screening tests
- 11. EVIDENCE OF HEMOLYSIS •LOW RBC SURVIVAL WITH CHROMIN TAGGING STUDY • UNCONJUGATED BILLIRUBIN • PLASMA HB • DECREASED SERUM HAPTOGLOBIN • COOMB’S TEST IS USED TO DETECT ANTIBODIES THAT ACT AGAINST THE SURFACE OF RBC
- 12. EVIDNECE OF ERYTHROPOIESIS •POLYCHROMASIA • INCREASED RETICULOCYTE • SHIFT MACROCYTOSIS • HYPERCELLULAR BM
- 13. PERIPHERAL BLOOD FINDINGS •Peripheral smear evaluation is the most important investigation in hemolytic anemias • The following morphological findings alone or in combination are suggestive of hemolysis : Polychromatophilia, nucleated red cells, thrombocytosis and neutrophilia with mild shift to left • Red cell morphologic abnormalities provide a clue to underlying disorder. Some are Spherocytes, Sickle cell, Target cells, Schistocytes (fragmented red cells, helmet cells, traingular cells) and acanthocytes
- 14. PS WITH ROMANOWSKYSTAIN SHOWING POLYCHROMATIC CELLS WHICH ARE BASOPHILIC BECAUSE OF INCREASED RNA CONTENT AND ARE LARGER THAN NORMAL RBCS AUTOIMMUNE HEMOLYTIC ANEMIA:NUMEROUS SPHEROCYTES,SMALL ROUND RBCS LACKING CENTRAL PALLOR
- 15. BONE MARROW FINDINGS •COMPENSATORY TO HEMOLYSIS • Erythroid hyperplasia of bone marrow- Erythroid hyperplasia with normoblastic reaction. Reversal of M:E ratio • Reticulocytosis – Increase variably • Mild (2-10%)- Hemogobinopathies • Moderate to marked (10-60%) oImmune hemolytic anemias oHereditary spherocytosis oG6PD deficient states
- 16. BONE MARROW BIOPSYIN A PATIENT WITH HEMOLYTIC ANEMIA:ERYTHROID HYPERPLASIA IS SEEN WITH A REVERSAL OF MYELOID TO ERYTHROID RATIO OF 1:4
- 17. CLASSIFICATION OF HEMOLYTICANEMIAS • HEREDITARY CAUSES • Inherited genetic defects 1. Red cell membrane defects – Heriditary spherocytosis, Hereditary elliptocytosis 2. Enzyme deficiencies • a. Hexose monophosphate shunt enzyme deficiencies – G-6-PD deficiency • b. Glycolytic enzyme deficiency – Pyruvate kinase deficiency, Hexokinase deficiency • Hemoglobin abnormalities 1. Deficient globin synthesis – Thalassemia 2. Structurally abnormal globins – Sickle cell disease
- 18. • ACQUIRED CAUSES •Acquired Genetic defects 1.Deficiency of phosphatidylinositol – linked glycoproteins – Paroxysmal nocturnal hemoglobinuria • Antibody mediated destruction • Hemolytic disease of newborn • Transfusion reactions • Autoimmune disorders • Drug – induced
- 19. • Mechanical trauma 1.Microangiopathichemolytic anemias – Hemolytic uremic syndrome, TTP, DIC 2.Cardiac traumatic hemolysis – Defective cardiac valves 3.Repetitive physical trauma – Marathon running, Karate chopping • Infections of RBC – Malaria, Babesiosis • Toxic chemical injuries – Snake venum, Lead poisoning, Clostridial sepsis • Membrane lipid abnormalities – Abetalipoproteinemia, Severe liver disease • Sequestration – Hypersplenism
- 20. AN APPROACH TO HEMOLYTICANEMIAS
- 21. HEREDITARY SPHEROCYTOSIS • Hereditaryspheroytosis is an inherited hemolytic anemia resulting from red cell mebrane defect leading to microspherocytosis, splenomegaly and jaundice
- 22. ETIOPATHOGENESIS • Spectrin deficiencyis the most common abnormality • Mutation of b spectrin gene and point mutations affect the binding of spectrin to protein 4.1 • The gene mutations that cause hereditary spherocytosis cause red blood cells to have an abnormal, spherical shape with decreased flexibility. The misshapen red blood cells are called spherocytes. The spherocytes are taken out of circulation and sent to the spleen to be destroyed (hemolysis). • This results in a shortage of red blood cells in the blood, and too many in the spleen
- 23. CLINICAL FEATURES • Seenall over the world • Autosomal dominent with variable penetrance • M=F ; present in neonate, childhood or adulthood • Intermittent jaundice is usual presentation • O/E- splenomegaly is a constant feature • Gall stones (pigment type) • Chronic leg ulcers (rare)
- 24. LAB FINDINGS • Microspherocyteswhich are small dense rbc without pallor • MCV- Normal • Reticulocytes- Increased •Bone marrow- Erythroid hyperplasia with normoblastic reaction • S. bilrubin- Increased (unconjugated ) • U. bilrubin – Increased • Fecal stercobilinogen- increased • S. haptoglobins- Reduced
- 25. OTHER DIAGNOSTIC TESTS •Osmotic fragility test- shift of curve to right • Incubated osmotic fragility test • Glycerol lysis test – Increased (rate of lysis) • Flow cytometry based on EMA (Eosin5-malemide)- lower in HS ( mean fluoroscent intensity of EMA tagged cells)
- 26. HEREDITARY ELLIPTOCYTOSIS • Groupof anemias characterised by the presence of elliptical or oval RBCs in the peripheral blood. Such cells should be more than 25% • Autosomal dominent disorder • Membrane protein abnormalities like a b-spectrin defect, structural defects or deficiency of protein 4.1 lead to elliptical shape of rbcs. membrane dysfunction and mild hemolysis
- 27. Clinically patient areasymptomatic and mild hemolytic anemia is fully compensated in most cases • Case is diagnosed incidentally when the blood film is examined for other ailment • Periperal smear demonstrates presence of elliptocytes ( cigar shaped ) which vary from 20-90% of cells. Osmotic fragility normal
- 28. Hereditary pyropoikliocytosis • Hereditarypyropoikliocytosis is a rare hemolytic anemia • There is a defective spectrin gene transmitted by one parent and also an elusive thalassemia like defect of spectrin synthesis inherited from normal parent • This results in a compound inheritance in which a spectrin abnormality is superimposed upon spectrin deficiency
- 29. Stomatocytosis • Stomatocytes arered cells with a slit like central pallor and • these are uniconcave/bowel shape in wet suspension • Normal individual <5% • Hereditary stomatocytosis >30% • Accquired stomatoctosis 5-50%
- 30. MANAGEMENT • Folate therapy •Red blood cell transfusions may be required in severe cases of anemia, particularly in the first years of life or during infections and pregnancy • If red blood cell transfusions are needed repeatedly, iron chelating therapy may be required to reduce iron overload. • Regular monitoring for anemia and gallstones is advised.
- 31. MANAGEMENT • Removal ofthe spleen (SPLENECTOMY) is usually only performed in severe HS or in moderate to severe cases with significant anemia and gallstone complications. • Splenectomy is not recommended in cases of mild HS except in specific cases.
- 32. ENZYMOPATHIES:GLUCOSE 6 PHOSPHATE DEHYDROGENASEDEFICIENCY • Glucose6-phosphate dehydrogenase is the first enzyme in the hexose monophosphate shunt pathway (HMP) which protects red cells from oxidant injury • Deficiency of G6PD may result in episodes of hemolysis following certain drug intake or chemical exposure or infection • G6PD deficiency is a sex linked disease. Its prevalance is higher in tropical eastern countries. Prevalance is higher in kurdish jews(60-70%) and lower in japan (1%)
- 33. Clinical and hematalogicalpresentation • Acute hemolytic anemia- Occurs following exposure to drugs like primaquine, infections like pneumonia, typhoid and oxidative chemicals. • CF- appears 1-3 hours after drug adiministration. Sudden development of pallor, passage of dark urine, jaundice and severe backache • Chronic non-spherocytic anemia- There is moderately severe enyme deficiency, hemolysis continues throughout life. Seen in neonatal period.
- 34. • Neonatal hyperbilrubinimia-Jaundice, • kernicterus • Favism- Common in children caused by consumption of fava beans. • Resulting in acute severe hemolysis within few hours . • CF-headache, fever, chills and back pain.
- 35. Diagnostic tests- 1. Peripheralblood film evaluation, history and biochemical finding- • Moderate anisopoikliocytosis with polychromatophilia • Microspherocytes and bite cell ( removel of heinz bodies) • Reticulocytosis (20-50%) • Hemogobinuria and increase urobilinogen in urine 2. Screening tests for G6PD deficiency are • Methemaglobin reduction test (MRT) • Ascorbate –cyanide test • Fluooscent spot test • Dye decolourisation test 3. Quantitative G6-PD assay and DNA analysis by PCR
- 36. Pyruvate kinase deficiency •This is the second common enzyme deficiency involving the glycolytic pathway of red cell metabolism. • Autosomal recessive conditon • Pyruvate kinase has 2 isoenzymes- PK-L ( Liver) and PK-M ( Muscles). • There is accumulation of G-3-P, and 2,3-DPG and glucoseClinical features • Neonatal jaundice to compensated hemolytic process. • Pallor , jaundice, gall stones and/or splenomegaly may be present
- 37. • Hematological findings •moderate anemia with reticulocytosis. Peripheral smear demostrates- Presence of prickle cells ( red cells having sharp thorn like projections), a few echinocytes and tailed poikliocytes
- 38. Pyrimidine 5 nucleotidasedeficiency: • Characterised by the presence of marked basophilic stippling of RBCs and echinocytes • Clinically, Mild spleomegaly wih intermittent jaundice
- 39. HEMOGLOBINOPATHIES The Thalassemias • Thalassemiasyndrome are autosomal recessive disorders • Thalassemia results from defects in the rate of synthesis of a or b chains, lead to reduced hemoglobin production and accumulation of a or b chains • Thalassemia is considered to be quantitative hemogolobinopathy, since no structural abnormal hb is synthesised
- 40. • Pathogenesis • Imbalancedsynthesis of α & β chains • Decreased total RBC Hb production • Ineffective erythropoiesis • Chronic hemolytic process • Systemic iron overload
- 41. • LAB FINDINGS •Microcytic Hypochromic anemia(2-3g/dl) • Decreased MCV , MCH , MCHC • Decreased Osmotic Fragility. • Increased serum uric acid • Anisopoikilocytosis • Lab Findings- BM Normoblastic erythroid hyperplasia • Increased macrophages • Inclusion bodies in normoblasts
- 45. Sickle cell disorders •Sickling syndromes are characterized by the presence of HbS which imparts sickle shape to red cells in a state of reduced oxygen tension • HbS is prevalant in Africa, Mediterranean countries and India. InIndia, seen common in tribals and in ethnic groups of MP, Orissa, AP, Maharashtra (vidharba region), TN (chetti tribes) and Kerala • There is high prevelance of HbS in areas endemic to malaria falciparum
- 46. • Genetics – •Sickle mutation is caused by substitution of valine in place of glutamic acid in the 6th position (b6 glu-val) ofb-chain • Mutation results in clinical presentation • 1. Sickle cell anemia- HbS-HbS, Homozygous state • 2. Sickle cell trait - HbA-HbS, heterozygous state • 3.Sickle cell disease- Refer to all diseases with HbS in combination with – normal (HbA), abnormal gene of b- thalassemia, a-thalassemia, HbD, HbE, HbC,HbQ
- 47. Pathophysiology of vascularocclusion and hemolysis • Polymerisation of deoxygenated HbS is the primary event in the pathogenesis of the disease • Red cell containing HbS pass through microcirculation of spleen – various cycles of sickling and desickling – Irreversible sickeled RBCs – Extravascular hemolysis in spleen – Vascular stasis – vascular occlusion – splenic infarcts – hyposplenism (lead to infection)and autosplenectomy
- 48. Clinical features • Delayin puberty, growth and development • Recurrent leg ulcers ,Avascular necrosis of femur head • Dactylitis ( Hand –Foot syndrome ) • Pneumonia, meningitis, Osteomylitis • Jaundice and liver enlargement ,Pigment gall stones • Acute abdominal pain ( infarcts of abdominal viscera) ,Priapism • Acute chest syndrome (fever, chest pain, leucocytosis, appearance of pulmonary infilterate with sickle anemia) • Sickle retinopathy- Salmon patches- intra retinal hemmorhages
- 49. Crisis in sicklingsyndrome • 1. Sickling crisis ( vaso-occlusive crisis) • 2. Hemolytic crisis • 3. Aplastic crisis • 4. Sequestration crisis
- 50. Sickle cell trait •Sickle cell trait usually do not manifest any clinical findings • Hemoglobin varies from 11-13 gm/dl • Red cells are normocytic normochromic and very target cells and mild degree of anisopoikliocytosis • Clinical and hematological picture is milder in comparison to HbSS state • Diagnosis is confirmed by Hb electrophoresis, HPLC and sickling test
- 51. Hematological findings – •Anemia- moderately severe anemia with Hb 5- 10 gm • PBF demonstrates – Red cells- Normocytic normochromic to mildly hypochromic • Moderate to severe degree of anisopoikliocytosis. • Sickle cells, target cells, ovalocytes, • Polychromtophila with nucleted RBCs. • Howell-jolly bodies alo seen • TLC- Mildly elevated ; Platlets- Increased • Reticulocytosis- 3%-10% • Bone marrow- Erythroid hyperplasia with normoblastic reaction
- 52. SICKLING TESTS- Presenceof HbS demostrated by using • reducing agent like 2% sodium metabisulphite • SICKLING SOLUBILITY TEST • Hb electrophoresis- Hb electrophoresis can be carried out on cellulose acetate membrane (pH8.9) or starch agarose (pH 8.6). HbS is a slow moving Hb as compared to HbA and HbF. However, electrophoretic mobility of HbD/HbQ india is similar to HbS , therefore sickling test is essential to differentiate .
- 53. 3. HPLC (HIGHPERFORMANCE LIQUID CHROMATOGRAPHY) • On HPLC, HbS has a retention time of 4.40 to 4.50 min, while HbD punjab is 4.50-4.15 min. HbSS/HbSA- In HbSS, major abnormal Hb is HbS constituting 70-90% of total Hb, HbF is 10-30% but HbA is nil. This differentiates homozygous state from heterozygous state, since the latter demonstrates 2 bands of HbS and HbA • HPLC is a sensitive method for confirmation of HbS
- 54. Management of sicklecell anemia Is usually aimed at 1. Avoiding pain episodes, 2. Relieving symptoms and 3. Preventing complications. • Treatments might include medications and blood transfusions. • For some children and teenagers, a stem cell transplant might cure the disease
- 55. Medications 1. HYDROXYUREA (DROXIA,HYDREA, SIKLOS). • Daily hydroxyurea reduces the frequency of painful crises and might reduce the need for blood transfusions and hospitalizations. It can also increase risk of infections. C/I pregnancy. 2. L-GLUTAMINE ORAL POWDER (ENDARI). • The FDA recently approved this drug for treatment of sickle cell anemia. • It helps in reducing the frequency of pain crises
- 56. • CRIZANLIZUMAB (ADAKVEO). •The FDA recently approved this drug for treatment of sickle cell anemia. Given IV, it helps reduce the frequency of pain crises. • Side effects can include nausea, joint pain, back pain and fever. • 4) VOXELOTOR (OXBRYTA). • The Food and Drug Administration (FDA) recently approved this oral drug to improve anemia in people with sickle cell disease. • Side effects can include headache, nausea, diarrhea, fatigue, rash and fever
- 57. • 5) PAIN-RELIEVINGMEDICATIONS. • Level of Pain • Suggested Medications • Mild pain Non-opioid ± adjuvant • Moderate pain Weak opioid (or low dose of strong opioid) ± non-opioid ± adjuvant • Severe pain Strong opioid ± non-opioid ± adjuvant
- 58. Preventing infections Children withsickle cell anemia might receive penicillin between the ages of about 2 months old until at least age 5. • Adults who have sickle cell anemia may need to take penicillin throughout their lives, if they've had pneumonia or surgery to remove the spleen. VACCINES recommended childhood vaccinations vaccines against pneumonia and meningitis and an annual flu vaccines.
- 59. Surgical and otherprocedures • Blood transfusions • Stem cell transplant. Also known as bone marrow
- 60. PAROXYSMAL NOCTURNAL HEMOGLOBINURIA Acquired Clonalcell disorder • Somatic mutation in hematopoietic stem cell • Defect in glycosyl– phosphatidyl inositol (GPI) molecule embedded in cell membrane • GPI linked proteins – decay accelerating factor (CD 55), Inhibitor of reactive lysis(CD 59)-Prevents activation of complement
- 61. LAB FINDINGS • .Anemia • Thrombocytopenia • Hemosiderinuria • Positive sucrose hemolysis test • Positive Ham’s test • Normal Osmotic fragility • SUCROSE HEMOLYSIS TEST Screening test Patient’s blood incubated in sucrose solution Sucrose promotes binding of complement to RBC Hemolysis • HAM’S TEST(Acidified serum lysis test) Patient’s RBCs are exposed at 37°C to action of normal / patient own serum suitably acidified to optimal pH for lysis( activate alternate pathway) 10 – 50 % of total RBC ---- Lysis FLOW CYTOMETRY
- 63. IMMUNE MEDIATED HEMOLYTICANEMIA RBC’s + IgGAb Pass through the spleen Fc receptor of spleen macrophages attracts Fc portion of Ab – RBC complex Phagocytosis of RBC by macrophage Extravascular hemolysis
- 66. WARM AIHA • Individualsproduce Ab against their own erythrocyte Ag (autoantibodies) • Abreact with red cell Ag best at 37oc. • Lab findings PBS – Normocytic normochromic anemia- Reticulocytosis- Spherocytes, - Schistocytes, Polychromasia, NRBC’s- Neutrophilia- Platelet - normal or decreased Bone marrow- Normoblastic erythroid hyperplasia - Erythrophagocytosis Other tests- Direct Coombs’ test (DAT)- positive
- 68. COLD AIHA • 1.Associated with IgM Ab which fixes complement & is reactive below 32o • First indication of the presence of unsuspected cold agglutinins is blood counts. RBC count is inappropriately decreased for Hb% • MCV is falsely elevated (due to agglutination) • PCV is falsely low • MCH & MCHC are falsely elevated • • Visible autoagglutination can be observed in tubes of anticoagulated blood as the blood cools to room temperature. When RBC indices go haywire think of the possibility of cold agglutinin disease
- 70. PAROXYSMAL COLD HEMOGLOBINURIA uLab findings a) Between the attacks - peripheral blood is normal except for anemia b) During the attack – sharp drop in Hb c)DAT – weakly +ve with anticomplement antisera- Ab are not detected d) Indirect coomb’s test may be +ve ,if performed in cold e) Donath– Landsteiner test DONATH LANDSTEINER(D-L) Test PATIENT’S WHOLE BLOOD INCUBATE FOR 30 MIN AT INCUBATE FOR 30 MIN AT CONTROL 370 C TEST 370 C Interpretation 40 C 370 C Centrifuge: Observe plasma for presence of hemolysis D-L antibodies present No hemolysis NO D-L antibodies present No hemolysis Hemolysis No hemolysis
- 72. AUTOIMMUNE HEMOLYTIC ANEMIA •❑ Hemolytic anemia induced by immunization of an individual with RBC Ag’s from another individual . • Eg: 1. Hemolytic transfusion reactions • 2. Hemolytic disease of new born • DRUG INDUCED AIHA It is the result of an immune mediated hemolysis precipitated by ingestion of certain drugs. • MECHANISM: 1. 2. 3. 4. Drug adsorption (hapten type) Immune complex formation Autoantibody induction Membrane modification Haemolytic uremic syndrome MALARIA Other conditions where hemolysis is seen ➢ Disseminated malignancy ➢ Leukemia ➢ Malignant lymphomas ➢ Renal failure ➢ Liver disease ➢ Rheumatoid arthritis ➢ Megaloblastic anemia