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Immunoelectrophoresis
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- 3. Immunoelectrophoresis isa type of Antigen –Antibody interaction. Antibodies are secreted inside the human body when the person encounters with an Antigen. Immunoelectrophoresis is the combination of electrophoresis and double immune diffusion It is a diagnostic technique for confirming the presence of different Antibodies in the blood serum. INTRODUCTION
- 4. Agarose gelis used as a supporting medium for electrophoresis and diffusion. Electrophoresis results in separation of Antigen according to their charge. When Antibodies are added to the electrophoresed gel the diffusion started. Wherever the antigen interacts with the antibody it will result in formation of a precipitation line. This is of two types: Quanlitative: Immunoelectrophoresis Quantitative: Rocket electrophoresis PRINCIPLE
- 5. In immunoelectrophoresis,the antigen mixture is first electrophoresed to separate its components by charge. Troughs are then cut into the agar gel parallel to the direction of the electric field, and antiserum is added to the troughs. Antibody and antigen then diffuse toward each other and produce lines of precipitation where they meet in appropriate proportions. METHODOLOGY
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- 8. Immunoelectrophoresis isused in clinical laboratories to detect the presence or absence of proteins in the serum. A sample of serum is electrophoresed, and the individual serum components are identified with antisera specific for a given protein or immunoglobulin class. This technique is useful in determining whether a patient produces abnormally low amounts of one or more isotypes, characteristic of certain immunodeficiency diseases. It can also show whether a patient overproduces some serum protein, such as albumin, immunoglobulin, or transferrin. APPLICATIONS
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