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Intracellular accumulations
- 1. INTRACELLULAR ACCUMULATIONS Presented by Dr.Vamshikrishna Dept. of Pathology
- 3. Intracellular accumulationof substances in abnormal amounts can occur within the cytoplasm (especially Lysosomes) or nucleus of the cell. Intracellular accumulation of the substance in - Mild degree causes Reversible Cell Injury. - Severe degree results in Irreversible Cell Injury.
- 4. Abnormal INTRACELLULAR ACCUMULATIONS canbe divided into 3 groups: Accumulation of Normal constituents of cell metabolism • LIPIDS • PROTEINS • CARBOHYDRATES Accumulation of Abnormal constituents produced due to abnormal metabolism • CERAMIDES • SPHINGOMYELIN • AMYLOPECTIN etc… Accumulation of pigments • ENDOGENOUS PIGMENTS (MELANIN, LIPOFUSCHIN) • EXOGENOUS PIGMENTS (INJESTED, INJECTED PIGMENTS)
- 5. INTRACELLULAR ACCUMULATION OFFAT FATTY LIVER: Liver is the commonest site for accumulation of fat because it plays central role in fat metabolism. Depending upon the cause and amount of accumulation of fat, fatty change may be Mild and reversible Severe producing irreversible cell injury and cell death.
- 6. ETIOLOGY Fatty changein the liver may result from one of the two types of causes: 1. Conditions with excess fat These are conditions in which the capacity of the liver to metabolize fat is exceeded E.g. i) Obesity ii) Diabetes mellitus iii) Congenital hyperlipidaemia
- 7. 2. Liver celldamage These are conditions in which fat cannot be metabolized due to liver cell injury e.g. 1- Alcoholic liver disease (most common) 2- Starvation 3- Protein calorie malnutrition 4- Chronic illnesses (e.g. tuberculosis) 5- Acute fatty liver in late pregnancy 6- Hypoxia (e.g. anaemia, cardiac failure)
- 8. 7- Hepatotoxins (e.g.carbon tetrachloride, chloroform, ether, aflatoxins and other poisons) 8- Drug-induced liver cell injury (e.g. administration of Methotrexate, Steroids, CCl4, Halothane Anaesthetic, Tetracycline etc) 9- Reye’s syndrome
- 9. PATHOGENESIS Mechanism offatty liver depends upon the stage at which the etiologic agent acts in the normal fat transport and metabolism. Hence, pathogenesis of fatty liver is best understood in the light of normal fat metabolism in the liver.
- 10. Lipids as freefatty acids enter the liver cell from either of the following 2 sources: From diet as chylomicrons (containing triglycerides and phospholipids) and as free fatty acids. From adipose tissue as free fatty acids. In liver a small part of fatty acids is also synthesized from Acetate.
- 11. In Normal FatMetabolism FREE FATTY ACIDS From diet From adipose tissue From Acetate. TRIGLYCERIDES CHOLESTEROL, PHOPHOLIPIDS, KETONES Lipoproteins production . Lipoproteins are released from the liver cells into circulation as plasma lipoproteins ‘LIPID ACCEPTOR PROTEIN’.
- 12. In Fatty Liver, Intracellular accumulation of triglycerides occurs due to defect at one or more of the following 6 steps in the normal fat metabolism. 1. Increased entry of free fatty acids into the liver. 2. Increased synthesis of fatty acids by the liver. 3. Decreased conversion of fatty acids into ketone bodies resulting in increased esterification of fatty acids to triglycerides. 4. Increased glycerophosphate causing increased esterification of fatty acids to triglycerides. 5. Decreased synthesis of ‘lipid acceptor protein’ resulting in decreased formation of lipoprotein from triglycerides. 6. Block in the excretion of lipoprotein from the liver into plasma.
- 13. Increased entry offree fatty acids into the liver FREE FATTY ACIDS
- 14. Increased synthesis offatty acids by the liver. Acetate fatty acids
- 15. Decreased conversion offatty acids into ketone bodies resulting in increased esterification of fatty acids to triglycerides. FREE FATTY ACIDS TRIGLYCERIDE PRODUCTION Decreased conversion to Ketone bodies
- 16. Increased glycerophosphate causingincreased esterification of fatty acids to triglycerides FREE FATTY ACIDS INCREASED TRIGLYCERIDE PRODUCTI Increased glycerophosphate activity (i.e. Esterification)
- 17. Decreased synthesis of‘lipid acceptor protein’ resulting in decreased formation of lipoprotein from triglycerides Low lipid acceptor protein Decreased formation of lipoprotein from triglycerides
- 18. Block in theexcretion of lipoprotein from the liver into plasma Lipoprotein produced in liver Block in the excretion of lipoprotein LIPOPORTEINS ACCUMULATES IN LIVER AND CAUSES FATTY LIVER
- 19. In most casesof fatty liver, one of the above mechanisms is operating. But liver cell injury from chronic alcoholism is multifactorial as follows: i) Increased lipolysis ii) Increased free fatty acid synthesis iii) Decreased triglyceride utilization iv) Decreased fatty acid oxidation to ketone bodies v) Block in lipoprotein excretion
- 20. Even asevere form of fatty liver may be reversible if the liver is given time to regenerate and progressive fibrosis has not developed. For example, intermittent drinking is less harmful because the liver cells get time to recover; similarly a chronic alcoholic who becomes teetotaler the enlarged fatty liver may return to normal if fibrosis has not developed.
- 21. MORPHOLOGIC FEATURES Grossly, the liver in fatty change is enlarged with a tense, glistening capsule and rounded margins. The cut surface bulges slightly and is pale-yellow to yellow and is greasy to touch
- 22. PIGMENTS Pigments arecolored substances present in most living beings including humans. There are 2 broad categories of pigments: 1- ENDOGENOUS 2- EXOGENOUS
- 23. A. ENDOGENOUS PIGMENTS Endogenous pigments are either normal constituents of cells or accumulate under special circumstances E.g of endogenous pigments: Melanin, Alkaptonuria, Haemoprotein-derived Pigments, and Lipofuscin.
- 24. Melanin Melanin isthe brown-black, non-haemoglobin- derived pigment normally present in the hair, skin, mucosa at some places, choroid of the eye, meninges and adrenal medulla.
- 25. In skin,it is synthesised in the melanocytes and dendritic cells, both of which are present in the basal cells of the epidermis and is stored in the form of cytoplasmic granules in the phagocytic cells called the melanophores, present in the underlying dermis. Melanocytes possess the enzyme tyrosinase necessary for synthesis of melanin from tyrosine.
- 26. Various DisordersOf Melanin Pigmentation Cause: - 1. Generalised And Localised Hyperpigmentation - 2. Generalised And Localised Hypopigmentation
- 27. i) Generalised Hyperpigmentation: a)In Addison’s disease, there is generalized hyperpigmentation of the skin, especially in areas exposed to light, and of buccal mucosa.
- 28. b) Chloasma observedduring pregnancy is the hyperpigmentation on the skin of face, nipples, and genitalia and occurs under the influence of oestrogen. A similar appearance may be observed in women taking oral contraceptives.
- 29. c) Inchronic arsenical poisoning, there is characteristic raindrop pigmentation of the skin.
- 30. ii) Focal hyperpigmentation: a)Cäfe-au-lait spots are pigmented patches seen in neurofibromatosis and Albright’s syndrome. Cäfe-au-lait spots neurofibromatosis
- 31. b) Peutz-Jeghers syndromeis characterised by focal peri-oral pigmentation. c) Melanosis coli is pigmentation of the mucosa of the colon.
- 32. Iii) Generalised Hypopigmentation Albinism is an extreme degree of generalized hypo pigmentation in which tyrosinase enzyme is genetically defective and no melanin is formed in the melanocytes. Oculocutaneous albinos have no pigment in the skin and have blond hair, poor vision and severe photophobia. They are highly sensitive to sunlight. Chronic sun exposure may lead to precancerous lesions and squamous and basal cell cancers of the skin in such individuals
- 33. iv) Localised hypopigmentation: a)Leucoderma is an autoimmune condition with localized loss of pigmentation of the skin. b) Vitiligo is also local hypopigmentation of the skin and is more common. It may have familial tendency. c) Acquired focal hypopigmentation can result from various causes such as leprosy, healing of wounds, DLE, radiation dermatitis etc.
- 34. Melanin-like Pigments ALKAPTONURIA Thisis a rare autosomal recessive disorder in which there is deficiency of an oxidase enzyme required for break down of homogentisic acid; the latter then accumulates in the tissues and is excreted in the urine (homogentisic aciduria). The urine of patients of alkaptonuria, if allowed to stand for some hours in air, turns black due to oxidation of homogentisic acid.
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- 36. The pigmentis melanin-like and is termed ochronosis, first described by Virchow. It is deposited both intracellularly and intercellularly, most often in the periarticular tissues such as cartilages, capsules of joints, ligaments and tendons ochronosis
- 37. Haemoprotein-derived Pigments Haemoproteinsare the most important endogenous pigments derived from Hemoglobin, Cytochrome and their break-down products. In disordered iron metabolism and transport, Haemoprotein-derived pigments accumulate in the body. These pigments are - Haemosiderin, - Acid Haematin (Haemozoin) - Bilirubin - Porphyrins
- 38. 1. HAEMOSIDERIN Ironis stored in the tissues in 2 forms: Ferritin, which is iron complexed to apoferritin and can be identified by electron microscopy. Haemosiderin, which is formed by aggregates of ferritin and is identifiable by light microscopy as golden-yellow to brown, granular pigment, especially within the mononuclear phagocytes of the bone marrow, spleen and liver where break-down of senescent red cells takes place.
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- 40. Excessive storageof Haemosiderin occurs in conditions when there is increased break-down of red cells. This may occur due to: 1. Primary (idiopathic, Hereditary) Hemochromatosis : 2. Secondary (Acquired): - Chronic Haemolytic Anaemias (e.g. Thalassemia), - Sideroblastic Anemia, - Alcoholic Cirrhosis, - Multiple blood transfusions etc.
- 41. Effects of haemosiderinexcess are: a) Localised Haemosiderosis: - This develops whenever there is hemorrhage into the tissues. - With lysis of red cells, haemoglobin is liberated which is taken up by macrophages where it is degraded and stored as haemosiderin. A few examples are as under:
- 42. 1. Changing coloursof a bruise or a black eye are caused by the pigments like biliverdin and bilirubin which are formed during transformation of haemoglobin into haemosiderin. 2. Brown induration of the lungs as a result of small haemorrhages occurring in mitral stenosis and left ventricular failure. - Microscopy reveals the presence of ‘heart failure cells’ in the alveoli which are haemosiderin-laden alveolar macro phages.
- 43. b) Generalized (Systemicor Diffuse) Haemosiderosis: Systemic overload with iron may result in generalized haemosiderosis. There can be two types of patterns: Parenchymatous deposition occurs in the parenchymal cells of the liver, pancreas, kidney, and heart. Reticuloendothelial (RE) deposition occurs in the RE cells of the liver, spleen, and bone marrow
- 44. Causes for Generalizedor Systemic overload of iron may be as under: i) Increased RBC lysis: ii) Excessive intestinal absorption of iron: iii) Excessive dietary intake of iron:
- 45. 1: Increased RBClysis: In various forms of chronic haemolytic anaemia, there is excessive break-down of haemoglobin and hence iron overload. The problem is further compounded by treating the condition with blood transfusions (transfusional haemosiderosis) or by parenteral iron therapy. The deposits of iron in these cases, termed as acquired haemosiderosis, are initially in reticuloendothelial tissues but may eventually affect the parenchymal cells of the organs.
- 46. 2: Excessive intestinalabsorption of iron: A form of haemosiderosis in which there is excessive intestinal absorption of iron even when the intake is normal, is known as idiopathic or hereditary hemochromatosis. It is associated with much more deposits of iron than in cases of acquired haemosiderosis. Haemochromatosis is characterised by triad of features: - Pigmentary liver cirrhosis, - pancreatic damage resulting in diabetes mellitus - Skin pigmentation. (bronze diabetes.)
- 47. Skin pigmentation. (bronzediabetes.)
- 48. 3: Excessive dietaryintake of iron: African iron overload: Earlier it was thought that those rural South African communities who consumed alcohol brewed in ungalvanised iron vessels that served as a rich source of additional dietary iron might had caused hemosiderosis. Later it was found that a gene, ferroportin, which predisposes iron overload in such people of African descent and hence the name.
- 49. 2. ACID HAEMATIN(HAEMOZOIN) Acid haematin or haemozoin, also called malarial pigment, is a haemoprotein derived brown-black pigment containing haem iron in ferric form in acidic medium. Haematin pigment is seen most commonly in chronic malaria and in mismatched blood transfusions. Besides, the malarial pigment can also be deposited in macrophages and in the hepatocytes.
- 50. 3. BILIRUBIN Bilirubinis the normal non-iron containing pigment present in the bile. It is derived from porphyrin ring of the haem moiety of haemoglobin. Normal level of bilirubin in blood is less than 1 mg/dl Excess of bilirubin or hyperbilirubinaemia causes an important clinical condition called jaundice.
- 51. 4. PORPHYRINS Porphyrinsare normal pigment present in haemoglobin, myoglobin and cytochrome. Porphyria (vampire’s disease) refers to an uncommon disorder of inborn abnormality of porphyrin metabolism. It results from genetic deficiency of one of the enzymes required for the synthesis of haem, resulting in excessive production of porphyrins.
- 52. Lipofuscin (Wear andTear Pigment) Lipofuscin or lipochrome is yellowish-brown intracellular lipid pigment (lipo = fat, fuscus = brown). The pigment is often found in atrophied cells of old age and hence the name ‘wear and tear pigment’. It is seen in the myocardial fibres, hepatocytes, Leydig cells of the testes and in neurons in senile dementia. However, the pigment may, at times, accumulate rapidly in different cells in wasting diseases unrelated to ageing.
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