Lec 6 isotonic solutions

ISOTONIC SOLUTIONS
PHYSICAL PHARMACY I
LEC 6
ASSISTANT LECTURER
DR AHMAD A YOSEF
MSC PHARMACEUTICAL SCIENCES
1

ISOTONIC SOLUTIONS
"When two solutions have same osmotic pressure and salt
concentration are said to be isotonic solutions”. Iso (same)
and tonic (concentration )
.
Physiologically, solutions having the
isotonic solutions are
pressure as that of the body fluids when
same osmotic
separated by a biological membrane. Biological fluids
including blood and lachrymal fluid normally have an osmotic
pressure corresponding to that of 0.9% w/v solution of sodium
chloride. Thus 0.9% solution of sodium chloride is said to be
isotonic with the physiological fluids.
2

In addition, to carrying out pH adjustments, pharmaceutical
solutions that are meant for application to delicate membranes of
the body should also be adjusted to approximately the same
osmotic pressure as that of body fluids. Isotonic solutions cause no
swelling or contraction of the tissues with which they come in
contact, and produce no discomfort when instilled in the eye,
nasal tract, blood or other body tissues. Isotonic sodium chloride
is a familiar pharmaceutical example of such a preparation.
0.9% w/v solution of NaCl has essentially the same concentration
and osmotic pressure as the RBCs contents. Thus it is said to be
isotonic with blood, and is also known as " Physiological Saline
solution", or "Normal saline solution"
If RBCs are suspended in 0.9% solution of NaCl, they undergo no
change in their size and shape due to isotonicity.
3

HYPERTONIC SOLUTION
"A hyper-tonic solution is one that has greater concentration
than reference solution (i.e. RBCs Contents )
.
"
A hyper-tonic solution has greater osmotic pressure than that
of reference solution.
If RBCs are suspended in 2% w/v solution of NaCl (i.e.
hypertonic solution), then water present within the RBCs will
come out (i.e. due to osmosis, from dilute RBCs fluid to
concentrated hypertonic solution) into the surroundings to
dilute the NaCl solution (hypertonic solution). This exit of
water from RBCs causes their shrinkage and RBCs become
wrinkled in shape. This shrinkage of RBCs is known as
"Plasmolysis "
.
4

HYPOTONIC SOLUTION
"A hypo tonic solution is one that has lower concentration than
reference solution (i.e. RBCs contents )
.
A hypo-tonic solution has lower osmotic pressure than that of
reference solution.
If RBCs are suspended in 0.1 % w/v solution of NaCl (i.e.
hypotonic solution), then water from this solution will enter the
RBCs (i.e. due to osmosis, from dilute hypotonic solution to RBCs
fluid) to dilute the fluid within the RBCs causing their swelling,
which may later result in rupturing of RBCs and release of
haemoglobin. This rupturing of RBCs is known as "Haemolysis”
5

ISO-OSMOTIC OR ISOSMOTIC SOLUTIONS
"Solutions having the same osmotic pressure but
not necessarily the same concentration are said
to be iso-osmotic or isosmotic solutions .
"
E.g. 2% w/v solution of boric acid has the same
osmotic pressure as 0.9% w/v solution of NaCI but
not the same concentration. So, both solutions
are iso-osmotic but not iso-tonic.
6

The tonicity of solutions may
be determined by one of the
following two methods:
1) Haemolytic Method.
2) Colligative Method
8

HAEMOLYTIC METHOD:
The acting principle of this method is the observation of the
effect of various solutions of drugs on the appearance of RBCs
when suspended in those solutions. If, there is no change in
size and shape of RBCs when immersed in test solution on
observing with microscope, then this solution is isotonic to the
blood.
This method can be made more accurate by using a hematocrit,
which is a centrifuge head in which a graduated capillary tube
is held in each of the two arms.
9

One capillary tube (tube A) is filled with blood diluted with
5 ml of 0.9% w/v NaCI (isotonic solution.)
The other capillary tube (tube B) is filled with blood
diluted with an equal volume i.e. 5ml of test solution.
Both tubes are centrifuged (i.e. rotated at high speed.)
After centrifuge, the blood cells are concentrated at one
end of the capillary tubes and the volume occupied by the
cells (i.e. PCV -Packed Cell Volume) is measured.
Finally, the PCV of test solution tube (tube B) is compared
with PCV of isotonic solution tube (tube A), and following
inferences are made.
10

RESULTS:
If PCV of test solution (tube B) is same as that of tube A,
then test solution is regarded as isotonic.
If RBCs volume (i.e. PCV) of tube is more than that of tube
A, then test solution is regarded as hypotonic solution
(increase in PCV is due to swelling of RBCs, which occurs in
case of hypotonic solution )
.
If RBCs volume (i.e. PCV) of tube is less than that of tube A,
then test solution is regarded as hypertonic solution
(decrease in PCV is due to shrinkage of RBCs, which occurs
in case of hypertonic solution )
.
11

COLLIGATIVE METHOD:
It has been determined that solutions having same tonicity
exhibit similar behavior with respect to their colligative
properties such as lowering of vapour pressure, depression in
freezing point, etc. Hence, tonicity of a solution may be
determined by determining its colligative properties.
For making isotonic solutions, the quantities of substances to be
added may be calculated by following methods:
Based on molecular concentration
Based on freezing point data
Based on sodium chloride equivalent (E) value
White-Vincent method
12

BASED ON MOLECULAR
CONCENTRATION
%1molecular concentration:
If one gram molecule (i.e. one gram molecular weight) of a
substance is dissolved in 100 ml of water, the resulting solution
will be of 1% molecular concentration. For example, the
molecular weight of boric acid is 62, so if 62 gms (i.e. one gram
molecular weight) of boric acid is dissolved in 100 ml of water,
the resulting solution will have 1% molecular concentration.
For non-ionizing substance, an aqueous solution having 1%
molecular concentration, depresses the freezing point to - 18.6
C°, and freezing point of plasma is - 0.52 C°. So, by using this
information, we can calculate the molecular concentration of
blood plasma as follows;
13

A depression of - 18.6 C° in freezing point of solution is due
to = 1% Molecular concentration
A depression of 1 C° in freezing point of solution is due to =
1 / - 18.6 % Molecular concentration
A depression of - 0.52C° in freezing point of plasma is due
to = 1 /- 18.6 x - 0.52
=
0.03% Molecular concentration
So, molecular concentration of plasma is 0.03%. Therefore,
any solution having the molecular concentration of 0.03%
will be isotonic with blood (having the same concentration -
which means isotonic )
.
14

The formula for calculating the w/v percent of ionizing and
non-ionizing substances required to make isotonic solutions
with blood plasma is as follows;
For non-ionizing substances
W / V % of substance required = 0.03% x Gram molecular
weight
For ionizing substances
W/V % of substance required = 0.03% x Gram molecular
weight / no. of ions yielded by the molecule
15

FIND THE PROPORTION OF BORIC ACID REQUIRED TO
MAKE A SOLUTION ISOTONIC. THE MOLECULAR WEIGHT OF
BORIC ACID IS 62, AND IT IS A NON-IONIZING SUBSTANCE
 Solution:
 By applying formulae for non-ionizing substances;
 W/V % of boric acid required to make isotonic solution =
0.03% x gram molecular weight
 =
0.03 x 62 = 1.86
 So, 1.86 gms of boric acid is required to make 100ml
isotonic solution.
16

FIND THE PROPORTION OF SODIUM SULPHATE
REQUIRED TO MAKE A SOLUTION ISOTONIC. THE
MOLECULAR WEIGHT BORIC OF SODIUM SULPHATE
IS 148, AND IT IS AN IONIZING SUBSTANCE.
By applying formulae for ionizing substances;
W/V % of ionizing substance required to make isotonic solution = 0.03% x
gram molecular weight / no. of ions
• Na2SO4 —> 2Na + SO4
So, total no. of ions produced by sodium sulphate = 3
W/V % of Na2SO4 required to make isotonic solution = 0.03% x gram
molecular weight / no. of ions
=
0.03
% x 148 / 3 = 1.48
S0, 1.48 gm of sodium sulphate is required to make 100ml isotonic
solution.
17

BASED ON FREEZING POINT DATA
Body fluids such as blood plasma and lachrymal secretions have a
freezing point of - 0.52 C° due to different solutes present in them.
0.9% solution of NaCI (isotonic solution) also has freezing point of
-
0.52 C . Hence, all solutions which freeze at - 0.52 C° will be isotonic
with these fluids.
Adjustment to the tonicity of solutions is simplified if the freezing
point of 1% solution of substance whose tonicity is to be adjusted (i.e.
unadjusted solution) and freezing point of 1% solution of adjusting
substance are known. The freezing points are usually expressed in
terms of 1% solution which can be noted from the reference table.
18

The quantity of the adjusting substance needed for
making the solution isotonic with blood may be
calculated from the general formula given below;
Amount of adjusting substance required = - 0.52 -a /b
Where,
a = freezing point of 1% solution of un-adjusted solution
b = freezing point of 1% solution of adjusting substance
19

Find the amount of sodium chloride required to render 1%
solution of cocaine hydrochloride isotonic with blood plasma. The
freezing point of 1% w/v solution of cocaine hydrochloride is
-
0.09 C°, and that of 1% NaCI is-0.576 C °
.
In this example,
Unadjusted solution (whose tonicity is to be adjusted) is 1% cocaine HCI
Adjusting substance is NaCI
Freezing point of 1% w/v solution of cocaine HCI (unadjusted solution) = a = - 0.09 C°
Freezing point of 1% w/v solution of NaCI (adjusting substance) = b = - 0.576 C°
Amount of adjusting substance required = - 0.52 -a /b
=
-
0.52
-
(
-
0.09
)
/
-
0.576
=
0.746 gms
Hence, by adding 0.746 gms of NoCI in 1% cocaine HCI solution, the final solution
becomes isotonic.
20

BASED ON SODIUM CHLORIDE
EQUIVALENT (E)
Sodium chloride equivalent i.e. (E) of a drug is defined as;
"The grams of Sodium chloride that will produce the same osmotic effect as
1 gm of that drug".
For example, potassium chloride has sodium chloride equivalent (E) value of
0.76gm NaCI / gm of KCI-. This means 0.76 gm of NaCI produce the same
osmotic effect as 1 gm of KCI.
To make a solution of a particular drug isotonic with blood plasma, the sodium
choride equivalent value (E) of that drug is noted from the reference table.
This E value is multiplied with the %age of the drug solution, and result so
obtained is subtracted from 0.9%. The difference in value so obtained is the
amount of NaCI needed to adjust the tonicity of the solution to isotonic
value.
Amount of NaCI required = 0.9% - {%age of solution x E)
21

Find the amount of sodium chloride needed to make a -
solution of 0.5% of KCI isotonic with blood plasma. Sodium
chloride equivalent value (E) of KCI is 0.76
.
Given solution (not isotonic) = 0.5% KCI
E value of KCI = 0.76 So, by applying formula,
Amount of NaCI required = 0.9 - (%age of drug x E)
= 0.9-(0.5 x 0.76
)
= 0.9 -0.38 = 0.52 gm —> Hence, 0.52 gm of NaCI
must be added in 0.5% KCI solution to make it
isotonic.
22

WHITE-VINCENT METHOD
This method involves the addition of water to the given
amount of drug to make isotonic solution, followed by the
addition of some other isotonic solution (e.g. 0.9% NaCI) to
make the final volume.
The volume of water that should be added in given amount of
drug to make isotonic solution is calculated by using following
formula;
V = W x E x 111.1
Where, V = volume of water needed to make isotonic solution
W = given weight of drug in grams
E = NaCI equivalent value of drug
111.1
= constant
23

MAKE 50ML ISOTONIC SOLUTION FROM 0.5 GM OF
BORIC ACID. E VALUE OF BORIC ACID IS 0.50
.
Given amount of boric acid = 0.5 gm
Required volume = 50 ml*
E value of boric acid = 0.50
Firstly, we calculate the amount of water that should be added in 0.5 gm of
boric acid to make isotonic solution by using formula,
V= W x E x 111.1
V = 0.5 x 0.5 x 111.1 = 27.8 ml
So, 0.5 gm of boric acid is dissolved in 27.8 ml of water to make isotonic
solution.
BUT
, final volume that is required is 50 ml. so, remaining 22.2ml (50 - 27.8 =
22.2) of some other isotonic solution (e.g. 0.9% NaCI) are added to make up
final 50 ml volume.
24

Lec 6 isotonic solutions

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