Download as PDF, PPTX
More Related Content
Similar to MASSON TRICHROME STAINS AND THEIR INTERPRETITIONS
MASSON TRICHROME STAINS AND THEIR INTERPRETITIONS
- 1. MASSON TRICHROME STAINAND THEIR INTERPRETATION - Tanushree Vishwakarma Roll no. - 209
- 2. • Introduction toMasson Trichrome stain • History • Principle • Applications • Preparation of tissue sample • Procedure • Interpretation • Advantages • Limitations and Challenges • Future Perspective OBJECTIVES -
- 3. Tri Chrome • TheTrichrome stain is one of the most commonly used special stains. • The Trichome stain is a general term for a number of techniques for the selective ability to differentiate tissue elements into three colours. • The three tissue structures demonstrated by three component dyes are- Nuclei, Cytoplasm and Extracellular collagen. WHAT IS TRICHROME STAIN?
- 4. • Different trichrometechniques include - Masson, Gomori one step, Martins scarlet blue and Mallory. Continued....
- 5. HISTORY • Masson trichromestain was developed by a French histologist, Paul Masson, in the early 20th century. • This staining method has evolved to enhance our understanding of tissue morophology and pathology,becoming a fundamental tool in histology.
- 6. PRINCIPLE- The less poroustissues are coloured by the smallest dye molecule ; whenever a dye of large molecular size is able to penetrate, it will always do so at the expense of the smaller molecule. Smaller molecular size dye Intermediate molecular size dye Large molecular size dye Larger dye molecule replaces smaller dye molecule
- 7. Solution for Massontrichrome technique-
- 8. PREPARATION OF TISSUESAMPLE- 1.Tissue fixation: Fixation is critical for preserving tissue morphology Fixatives like Formalin are used, they stabilize proteins, preventing enzymatic degradation, and allowing for better staining outcomes. 2. Embedding techniques: Embedding in paraffin or resin helps maintain tissue structure during sectioning; while paraffin is widely used, resin embedding is preferred for more delicate specimens due to its superior support. 3. Sectioning protocols: Tissue samples are sliced into thin sections (typically 4-6 micrometers) using a microtome, ensuring uniformity and enabling optimal dye penetration and staining consistency.
- 9. PROCEDURE FOR STAINING- Deparaffinize sections and take to water. Remove mercury pigment by iodine, sodium thiosulfate sequence. Wash in tap water. Stain nuclei by the Celestine blue-hematoxylin method. Differentiate with 1% acid alcohol. Wash well in tap water.
- 10. Continued….. Stain in acidfuchsin Solution a and keep it for 5 mins. Rinse in distilled water. Treat with phosphomolybdic acid Solution b for 5 mins. Drain Stain with methy blue Solution c for 2-5 mins. Rinse in distilled water Dehydrate through ascending grades of alcohol. Clear in xylene, mount in permanent mounting medium.
- 11. Collagen INTERPRETATION- Masson trichrome stainon a section of skin demonstrating collagen in the dermis Epidermis Dermis Blue or Green Collagen , Cartilage and Mucin Red Cytoplasm , Muscle and RBCs Blue/Black Nuclei
- 12. Common Applications ofMasson Trichrome technique Visualizing collagen Fibrosis assessment
- 13. Collagen 1.Visualizing Collagen- Epidermis Dermis Masson trichromestain on a section of skin demonstrating collagen in the dermis
- 14. 2. Fibrosis assesment- Alcoholiccirrhosis Fibrous tissue Alcoholic steatosis and fibrosis Dilated Cardiomyopathy(Myocyte hypertrophy and interstitial fibrosis)
- 15. ADVANTAGESOF MASSON TRICHROMEOVER OTHER STAINING TECHNIQUES - 1.Specificity for connective tissues- Masson trichrome staining highlights collagen and muscle fibres, making it superior for assessing connective tissue disorders compared to other staining methods that may lack this specificity. 2. Comprehensive Assessment- The ability to demonstrate multiple components in one staining process enhance diagnostic discernment in complex cases. 3. Clinical Relevance- Aids in research and has significant clinical implications.
- 16. 1.False Positive 2.Staining Artifacts 3.Technical Variability LIMITATIONS AND CHALLENGES- Occasionally, non-collagenous structures may be misidentified as collagen due to staining irregularities. Artifacts such as precipitates or uneven staining can arise from inadequate sample handling or improper reagent concentrations, complicating interpretation of result. Variability in tissue processing and staining conditions can lead to inconsistencies in results.
- 17. 11.Emerging Techniques 2. Innovationsin Staining Protocols1 FUTURE PERSPECTIVE- Advancements in staining technologies, such as autoomated staining systems and novel dyes,aim to enhance efficiency and specificity,paving the way for improved histological techniques. Continued research into optimized protocols could reduce variability and enhance reproducibility, facilitating a more standardised approach to tissue analysis. 3. Interdisciplinary Approaches1 Collaboration between fields like bioinformatics, pathology, and imaging science can lead to new methods .
- 18. CONCLUSION- Masson trichrome stainingprovides a valuable methodology for visualising and differentiating tissue components. It allows to assess connective tissue disorders. Continuous improvements and innovations in staining methodoligies will shape the future of histologicak research and clinical applications.
- 19. REFRENCES- • Ramdas Nayak,Histopathology techniques and its Management, 1st edition, 2018; pg no. 226-229 • Robbins & Cotran, Pathologically Basis Of Disease, edited by Manoj K. Singh and Vinay Kumar, 10th edition , 2018; pg no. 571,829,844
- 20.