MICROBIOLOGY
OF
DAIRY
PRODUCTS
PRESENTED BY
 RUFIA ABBAS
NUTRIENTS
Dairy Products like
milk contain
components
essential for human
body including
Proteins,
carbohydrates, fats,
water and vitamins.
Getting more
potassium reducing
salt intake can lower
bp and reducing risk
of heart attack.
MICROBIAL GROWTHS IN MILK
 Milk is also a good medium for the growth of ...
....microorganisms.
 Yeast, Moulds and a broad spectrum of bacteria
can grow in milk at different temperatures..
 The process to pasteurization is therefore used in
...order to eliminate these organisms.
Lactococc
us.
lactis
Coliform.
bacteria
Achromo
bacter
Bacillus.
subtilis
 First discovered by Louis Pasteur in
...1860s and was adopted for the
...processing of milk in 1895.
 Its objective is to eliminate disease
...causing bacteria from milk, though
...total number of bacteria is also very
...much reduced during this process.
 The chances of milk spoilage are
....reduced by this process.
METHODS OF PASTEURIZATION
1
• Low-Temperature-Longer-Time (LTLT)
• 65°c for 30 minutes, also called the Holding or Batch Method
2
• High-Temperature-Short-Time (HTST)
• Heat treatment of 72°c for 15 seconds is applied, followed by the rapid cooling to
below 10°c. Also called Continuous System or Flash Pasteurization.
3
• Ultra High Temperature (UHT)
• 149.5°c for 1 second or 93.4° c for 3 seconds.
FERMENTATION
 Metabolic Process that
...produce chemical changes
...in organic substrate
...through the action of
...enzymes.
 It’s primary means of
...producing adenosine
...triphosphate (ATP) by
...the degradation of
...organic nutrients
...anaerobically
Fresh milk is inoculated with the
appropriate bacteria, keeping it at
a temperature which favors
bacterial growth.
As the bacteria grow, they covert
milk sugar (lactose) to lactic acid
which can be detected by “Tart” or
“Sour taste”
The lower pH caused by
Lactic acid preserves the
milk.
LACTIC ACID FERMENTATION
Fermented Dairy Products
 Accumulation of Lactic acid from metabolism of Milk Sugar
(Lactose) produces Fermented Dairy Products.
 The difference in the flavor and aroma of various dairy
products are due to additional fermentation products.
 Many products are formed from microbial fermentation
process including butter, buttermilk, sour cream, kefir, yogurt
and many types of cheese.
Yogurt
 Yogurt is a variety of curd.
Whole, low fat, skim milks and
cram can be used to make yogurt.
 In the production of yogurt a
mixed culture of Streptococcus
thermophilus and lactobacillus
acidoplilus is added to the
pasteurized milk & incubated at 42-
46 °c.
Cheese
 Cheese consists of milk curds that
have been separated from liquid
portion the milk.
 The curdling of milk is done by
enzyme rennin and lactic acid
bacterial starter cultures.
 Cheese is produced in wide range
of flavors, textures and forms.
Skimmed Milk
• Fat content reduced to 0.5-2% by centrifugation.
• 10% skim milk solution prepared in tubes, is used for the
maintenance and propagation of lactic acid bacteria in
labs.
Malted Milk
It’s a powered gruel made from a mixture of malted
barley, wheat flour and evaporated whole milk. It comes
in two forms,
• Diastatic Malt
• Nondiastatic Malt
NON FERMENTED DAIRY PRODUCTS
Milk Borne Diseases
 Pathogenic organisms have been isolated from milk so milk can
serve as a carrier of diseases. These diseases include,
1. Typhoid Fever
2. Diphtheria
3. Scarlet Fever
4. Dysentery
5. Septic Sour Throat
 However this has become less common as milk sanitation has
improved and pasteurization is being more widely practiced.
Thank
You

Microbiology of Dairy Products

  • 1.
  • 2.
    NUTRIENTS Dairy Products like milkcontain components essential for human body including Proteins, carbohydrates, fats, water and vitamins. Getting more potassium reducing salt intake can lower bp and reducing risk of heart attack.
  • 3.
    MICROBIAL GROWTHS INMILK  Milk is also a good medium for the growth of ... ....microorganisms.  Yeast, Moulds and a broad spectrum of bacteria can grow in milk at different temperatures..  The process to pasteurization is therefore used in ...order to eliminate these organisms.
  • 4.
  • 5.
     First discoveredby Louis Pasteur in ...1860s and was adopted for the ...processing of milk in 1895.  Its objective is to eliminate disease ...causing bacteria from milk, though ...total number of bacteria is also very ...much reduced during this process.  The chances of milk spoilage are ....reduced by this process.
  • 6.
    METHODS OF PASTEURIZATION 1 •Low-Temperature-Longer-Time (LTLT) • 65°c for 30 minutes, also called the Holding or Batch Method 2 • High-Temperature-Short-Time (HTST) • Heat treatment of 72°c for 15 seconds is applied, followed by the rapid cooling to below 10°c. Also called Continuous System or Flash Pasteurization. 3 • Ultra High Temperature (UHT) • 149.5°c for 1 second or 93.4° c for 3 seconds.
  • 7.
    FERMENTATION  Metabolic Processthat ...produce chemical changes ...in organic substrate ...through the action of ...enzymes.  It’s primary means of ...producing adenosine ...triphosphate (ATP) by ...the degradation of ...organic nutrients ...anaerobically
  • 8.
    Fresh milk isinoculated with the appropriate bacteria, keeping it at a temperature which favors bacterial growth. As the bacteria grow, they covert milk sugar (lactose) to lactic acid which can be detected by “Tart” or “Sour taste” The lower pH caused by Lactic acid preserves the milk. LACTIC ACID FERMENTATION
  • 9.
    Fermented Dairy Products Accumulation of Lactic acid from metabolism of Milk Sugar (Lactose) produces Fermented Dairy Products.  The difference in the flavor and aroma of various dairy products are due to additional fermentation products.  Many products are formed from microbial fermentation process including butter, buttermilk, sour cream, kefir, yogurt and many types of cheese.
  • 10.
    Yogurt  Yogurt isa variety of curd. Whole, low fat, skim milks and cram can be used to make yogurt.  In the production of yogurt a mixed culture of Streptococcus thermophilus and lactobacillus acidoplilus is added to the pasteurized milk & incubated at 42- 46 °c.
  • 11.
    Cheese  Cheese consistsof milk curds that have been separated from liquid portion the milk.  The curdling of milk is done by enzyme rennin and lactic acid bacterial starter cultures.  Cheese is produced in wide range of flavors, textures and forms.
  • 12.
    Skimmed Milk • Fatcontent reduced to 0.5-2% by centrifugation. • 10% skim milk solution prepared in tubes, is used for the maintenance and propagation of lactic acid bacteria in labs. Malted Milk It’s a powered gruel made from a mixture of malted barley, wheat flour and evaporated whole milk. It comes in two forms, • Diastatic Malt • Nondiastatic Malt NON FERMENTED DAIRY PRODUCTS
  • 13.
    Milk Borne Diseases Pathogenic organisms have been isolated from milk so milk can serve as a carrier of diseases. These diseases include, 1. Typhoid Fever 2. Diphtheria 3. Scarlet Fever 4. Dysentery 5. Septic Sour Throat  However this has become less common as milk sanitation has improved and pasteurization is being more widely practiced.
  • 14.