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Nephlometry & turbidimetry
- 1. TURBIDIMETRY AND NEPHELOMETRY
- 2. TURBIDIMETRY AND NEPHELOMETRY Presented By - Mr. Shaise Jacob Faculty Nirmala College of Pharmacy Muvattupuzha, Kerala India Email â [email protected]
- 3. » Analysisof Suspensions NEPHELOMETRY â Measurement of scattered light ( opalescence) TURBIDIMETRY - Measurement of transmitted light ( turbidity ) » absorption, refraction & reflection -> transmitted Tyndall Effect .. Scattering of light-
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- 7. Turbidimetry âš ColorimetryNephelometry âš Fluorimetry
- 8. NEPHELOMETRY â concentration, uniform scattering, intensity of scatted light proportional to conc. measured at 90 0 TURBIDIMETRY â concentration, scattering not uniform, intensity of transmitted light measured at 180 0 Factors affecting measurements Concentration, Particle geometry, WL of incident light, RI difference
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- 11. Tungsten lamp Filters-Turbidimeter (blue filter or 530 nm) Nephelometer (visible filter) Sample cells Detectors (photometric) SOP Nephelometer: Flourimeter -> Nephelometer by using a visible filter as secondary filter
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- 13. Difference: Scattering ofradiation Difference: Absorption of radiation TURBIDIMETER Similarity: transmitted light measured measured at 180 0 COLORIMETER Similarity: transmitted light measured measured at 180 0
- 14. Difference: » emitted radiation measured » emitted radiation â longer WL than incident light FLOURIMETRY Similarity: Emergent radiation measured at 90 0 Difference: » Scattered radiation measured » emitted radiation â same WL as that of incident light NEPHELOMETRY Similarity: Emergent radiation measured at 90 0
- 15. APPLICATIONS Analysis ofwater clarity, conc. of ions Determination of CO 2 Determination of inorganic substances Sulphate â barium chloride Ammonia â Nesslers reagent Phosphorus â Strychine molybedate Biochemical Analysis 5. Quantitative Analysis â (ppm level)
- 16. 6. Miscellaneous Watertreatment plants, sewage work, refineries, paper industry 7. Atmospheric pollution smokes & fogs 8. Determination of mole. Wt of high polymers 9. Phase titration NEPHLOTURBIDIMETER Two detectors
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