Noreen moin

Noreen moin

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  Evaluation of resistancein wheat against Rhopalosiphum padi (L.) (Homoptera: Aphididae) Noreen Moin 05-arid-345 PMAS-Arid Agriculture University Rawalpindi
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  CONTENTS Introduction Materials and methods Seedlingbulk test Antibiosis test Tolerance test Results Seedling bulk test Antibiosis test Tolerance test Conclusion Recommendation
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  INTRODUCTION Aphids are consideras serious pests of crops (Wheat, Barely, Maize etc) Transmit virus (MMSV, BYDV) Persist through out the wheat season Reproduce Sexually Asexually
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  INTRODUCTION continue… Some importantspecies of aphids in Pakistan Bird cherry oat aphid (Rhopalosiphum padi (L)) Wheat grain aphid (Sitobion avenae) Grain aphid (Sitobion miscanthi) Corn leaf aphid (Rhopalosiphum maidis) Control strategies Insect pest management Benefits of IPM Environmentally sound method Economic
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  OBJECTIVES Evaluation of resistantvarieties of National Uniform Wheat Yeild Trials (NUWYT) Rain Fed (RF) against Bird cherry oat aphid by applying following tests Seedling bulk test Antibiosis test Tolerance test
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  MATERIALS AND METHODS Hostwas wheat 11 varieties of (NUWYT) Rain Fed (R.F) of year 2004-05. Experiments were conducted under controlled environment 28+2 oC, 40-70% RH and photoperiod of 16:8h Mass rearing was done in NARC by establishing the culture of Rhopalosiphum padi (L) Resistance was evaluated by Seedling bulk test Antibiosis test Tolerance test
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  SEEDLING BULK TEST Metaltrays measuring(51x35x9 cm) Eight rows of one cm depth 25 seeds per genotype was sown Ten aphids were released at seedling height of 5-6 cm Damage rating was recorded after 10-15 days
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  ANTIBIOSIS TEST Two seedswere sown in 7 cm diameter pot At height of 5-6 cm, each variety was thinned to one One adult aphid was released Covered with plastic cage having two side ventilation holes, 6 cm diameter and 30 cm height One nymph was allowed to grow until it becomes sexually mature Nymphs reproduced daily were removed and counted for each seedling
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  TOELRANCE TEST Seeds were sownas described in antibiosis test Seedlings were infested by aphids at the height of 5-6 cm Ten adults were maintained
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  RESULTS Seedling bulk test VarietiesNUWYT (RF) No. of seedlings Damage rating (DR) Nature of resistance PR-87 21 4 MR NR-241 17 4 MR SN-128 20 5 MR V-00BT004 16 5 MR V-00055 18 6 MR V-5 20 3 R NRL-2017 22 7 S NR-234 15 5 MR
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  RESULTS Seedling bulk testCONTI…. Varieties NUWYT (RF) No. of seedlings Damage rating (DR) Nature of resistance V-002467 15 4 MR DN-44 18 4 MR PR-83 25 5 MR Chakwal-97 14 7 S R = Resistant varieties with damage rating = 3 MR = Moderately Resistant varieties with damage rating = 4-6 S = Susceptible varieties with damage rating =7-9
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  RESULTS ANTIBIOSIS TEST Varieties NUWYT(RF) Average no. of nymphs Remarks PR-87 1.3 MF NR-241 1.3 MF SN-128 1.5 HF V-00BT004 1.3 MF V-00055 1.2 MF V-5 1.0 MF NRL-2017 0.9 MF NR-234 1.1 MF
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  Varieties NUWYT (RF)Average no. of nymphs Remarks V-002467 0.5 LF DN-44 1.2 MF PR-83 0.7 LF Chakwal-97 1.6 HF Wafaq-2001 1.3 MF RESULTS ANTIBIOSIS TEST CONTI.. LF = Least Fecund MF = Moderately Fecund HF = Highly Fecund
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  RESULTS TOLERANCE TEST Varieties NUWYT(RF) Average DR Remarks PR-87 3.8 MT NR-241 3.6 HT SN-128 3.6 HT V-00BT004 6.2 LT V-00055 4.4 MT V-5 3.6 HT NRL-2017 4.4 MT NR-234 5.4 MT
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  RESULTS TOLERANCE TEST CONTI.. VarietiesNUWYT (RF) Average DR Remarks V-002467 5.2 MT DN-44 4.6 MT PR-83 3.6 HT Chakwal-97 4.2 MT Wafaq-2001 4.6 MT HT = Highly Tolerant MT = Moderately Tolerant LT = Least Tolerant
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  3.8 3.6 3.6 6.2 4.4 3.6 4.4 5.4 5.2 4.6 3.6 4.2 4.6 0 1 2 3 4 5 6 7 Wheat varietiesNUWYT (RF) 2004-05 along with Wafaq 2001 AvgDamageRating PR-87 NR-241 SN-128 V-00BT004 V-00055 V-5 NRL-2017 NR-234 V-002467 DN-44 PR-83 Chakwal-97 Wafaq-2001
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  RECOMANDATIONS The use ofresistant varieties will remain the most logical and economical way of reducing damage of pests in wheat germplasm CONCLUSIONS In seedling bulk test V-5 was highly resistant In antibiosis test least fecund line was PR-83 In tolerance test four lines NR-241, SN-128, V-5 and PR-83 was found to be tolerant to aphid R.padi