Planaria 1

Planaria 1

• 1.
  S Tomomi Shore, ShawnCyran Biomedicine Program, Academies@Englewood March 5, 2015
• 2.
  What is aplanarian?• Classified in the Platyhelminthes phylum or “flat worms” • Carnivores • No anus and eats using pharynx in middle of its body • Has central nervous system Flat worm with ability to regenerate
• 3.
  What is aplanarian? Continued • Sexually reproduce and asexually reproduce through division • Hypotheses for regeneration: • Epimorphosis- neoblasts • Morphallaxis- morphogens give instructions • Polar Coordinate model- molecules act as “zip code”
• 4.
  S Retinoids (foundin animals) and carotenoids (found in plants) are two subgroups of vitamin A group. S Retinoids provide immune, inflammatory, genetic, and reproductive benefits and are important to cell growth and embryonic development. Retinol
• 5.
  Purpose/Hypothesis S Since VitaminA is essential to growth and maintenance of the body, we studied the effects of foods with varying amounts of vitamin A on Planarian regeneration. S We hypothesized that Planarians fed greater amounts of vitamin A would show greater regeneration compared to others fed less.
• 6.
  Vitamin A RetinoidFound in Foods Food Vitamin A per 100g Chicken Breast 50 IU Egg Yolk 1,442 IU Beef Liver 53,400 IU Turkey Liver 72,350 IU S We predicted that turkey liver would have the greatest growth while chicken breast would have the least because turkey liver has the most vitamin A, and chicken breast has the least.
• 7.
  Methods Feed planarians designated food one week before amputation Place (using pipette) Planarian on ice mountfor 40-80 seconds Put Planarian on coffee filter on petri dish to surgically amputate the head Remove coffee filter, place graph paper under petri dish
• 8.
  Before After Head Body Pictures
• 9.
  Results: Head PiecesGrowth Food Day 0 Day 1 Day 5 Day 6 Day 12 Turkey Liver 0 0.15833 3333 1.43333 3333 1.65 1.93333 333 Beef Liver 0 0.11666 6667 0.66666 6667 0.76666 6667 1.38666 6667 Egg Yolk 0 0 0.73333 333 0.93333 333 1.33333 33 Chicken Breast 0 0.25333 3333 1 1.01666 6667 1.2
• 10.
  Food Day 0Day 1 Day 5 Day 6 Turkey Liver 0 0.116666 667 1.916666 667 2.3 Beef Liver 0 0.063333 333 1.283333 33 1.283333 33 Egg Yolk 0 0.173333 33 0.84 1.79 Chicken Breast 0 0.1 0.716666 66 1.12 Results: Body Pieces Growth
• 11.
  Graphs: Heads Growth 0 0.5 1 1.5 2 2.5 Day0 Day 1 Day 5 Day 6 Day 12 Turkey Liver Beef Liver Egg Yolk Chicken Breast
• 12.
  Graphs: Bodies Growth 0 0.5 1 1.5 2 2.5 Day0 Day 1 Day 5 Day 6 Turkey Liver Beef Liver Egg Yolk Chicken Breast
• 13.
  Analysis S In boththe heads and bodies, the turkey liver groups had the greatest total growth compared to the others. S In both the heads and bodies, the chicken breast groups had the least total growth compared to the others. S The beef liver had a greater total growth compared to the egg yolk in heads, but egg yolk was greater than beef liver in bodies. This slight variation shows that these amounts of vitamin A were not large enough to show any noticeable differences.
• 14.
  Conclusion S Results confirmhypothesis S As we predicted, we found that the group of Planarians fed turkey liver grew the most and the Planarians fed chicken breast grew the least.
• 15.
  Acknowledgements