The document outlines an experiment focused on the plant breeder's kit, detailing the necessary tools and their specific purposes in plant breeding activities such as selfing and artificial crossing. Key items mentioned include magnifying glasses, forceps, scissors, needles, brushes, and weighing balances, each with defined sizes and uses. The kit is essential for conducting observations, managing pollen, and ensuring accurate labeling during breeding experiments.
Experiment No.1
PLANT BREEDER'SKIT
Prepared By:
Asit Prasad Dash
Assistant Professor
DEPARTMENT OF PLANT BREEDING AND GENETICS
INSTITUTE OF AGRICULTURAL SCIENCES
SIKSHA ‘O’ ANUSANDHAN (DEEMED TO BE UNIVERSITY), BHUBANESWAR, 751029
2.
Introduction:
The plant breederfrequently uses different
tools/ instruments and materials to carry out
selfing, artificial crossing and for taking field
observations.
Aim of the experiment-
To Study about the Plant breeder's kit and its
uses.
3.
1. Magnifying glaass/lense
Types/size:Small to medium size with handle
Purpose :To observe small flower buds. stigmatic surface and
dehiscence of anthers.
It ensures presence /absence of any pollen in
emasulated flower bud.
4.
Types/size :Medium tolong and pointed
Purpose :For emasculation and for holding stamens & anthers
during the operation.
2. Forceps
5.
Types/size : Smallto medium size with sharp edges
Purpose : Required for clipping or removal of unwanted parts
such as buds & awns etc.
3. Scissors
6.
Types/size :Long withplastic handle
Purpose :Required for opening of flower buds and separating
the flower parts.
4. Needles
7.
Types/size :Size 3or 4
Purpose :For collection of pollen and placing on the stigmatic
surface. It is very useful when a little quantity of
pollens are produced.
5. Brushes
8.
Types/size : Differentsizes of Parchment Paper bag,
brown paper bag, muslin cloth bags
Purpose :For covering flower bud or entire inflorescence or
whole plant during selfing or crossing (before and
after emasculation)
6. Bags
9.
Types/size :Small vial
Purpose:For Sterilizing forceps. Scissors, needles, brushes
and hands during emasculation and pollination.
7. Ethyl Alcohol
10.
Types/size : Cardboard,paper or tin tags
Purpose :Labelling flowers/plants in the field.
Information like names of male & female parents,
date of emasculation and date of pollinations may be
written on the labels.
8. Tags
Types/size :1-meter size
Purpose:Required for measuring plant height & dimensions
of field used for field experiment
10. Meter scale & measuring tape
.
Types/size :Standard size
Purpose: To note down daily observations in the field
regarding germination, flowering, morphological
description, plant height, weight of grains etc.
12. Field Book