Research – An Overview
R.C. Gupta
MD (Biochemistry)
NIMS, Jaipur
What is research?
No single definition
Defined in different ways
by different persons
Some definitions
A systematic effort to gain new knowledge
A careful investigation for new facts
Discovery of new facts and their interpretation
Revision of theories in the light of new facts
What is common is the word ‘new’
Research is ...
Discovery of new facts or knowledge
Adding new information to existing
knowledge
New practical application of
knowledge
Scientific research
Unlike other
disciplines,
scientific
research
doesn’t
depend
solely uponː
Mental
processes
Critical
thinking
Reasoning
Personal
opinions
Scientific research requires scientific
method
Scientific method generally has four
stepsː
Making an
observation
Forming a
hypothesis
Testing the
hypothesis
Reaching a
conclusion
1 2 3 4
Observation – Drug X decreases blood
pressure
Hypothesis – Drug X can be used to treat
hypertension
Testing – Give drug X to a group of hyper-
tensives and monitor blood pressure
Conclusion – Drug X is or is not useful in
treating hypertension
Observation – Drug X decreases blood
pressure
Hypothesis – Drug X is better than the
older drug Y in controlling blood pressure
Testing – Give drug X and drug Y to two
groups and monitor blood pressure
Conclusion – Drug X is or is not better
than drug Y in treating high blood pressure
Controlled study
Scientific research generally begins with
a research question and/or a hypothesis
A hypothesis is an assumption based on
some evidence
A research question is an unanswered
question
The researcher tests the hypothesis to
find out whether it is correct
To test the hypothesis, the researcher has
to use certain methods
A methodical study would test the hypo-
thesis and answer the research question
Frame the research
question
Form a hypothesis
Collect data to test
the hypothesis
Interpret data to
answer the question
Research methodology
Question
Search literature
Form hypothesis
Conduct experiments
Collect data
Interpret data
Share knowledge
New questions
arise
Merit of the research depends uponː
Selection of problem
The “research topic” is really a “problem”
which the researcher tries to solve
How important
the problem is
How useful the
solution provided is
Before
finalizing
the
problem,
look at
itsː
Relevance
Originality
Feasibility
Cost effectiveness
Ethical issues
Framing the research question
Success of research depends upon how
well the research question is framed
The question tells us whether the problem
is relevant, important and researchable
The research question should be specific
and precise
Research question – The PICO format
P
I
C
Outcome
O
Population
Intervention
Comparison
Population The study population
Intervention Treatment, procedure, test etc
Comparison The population for comparison
Outcome The effect of the intervention
The question should describe P, I, C & O
Is pain medication more effective than
aerobic stretching in controlling post-
operative pain in adult patients with
total hip replacement?
A sample research question
P – Adults with total hip replacement
I – Pain medication
C – Aerobic stretching
O ̶ Post-operative pain
Research question – Criteria
F – Feasible
I – Interesting
The
research
question
should
meet
FINER
criteria
N – Novel
E – Ethical
R – Relevant
A research problem, framed as a question,
is the precursor to hypothesis
Hypothesis is a paring down of the problem
into something verifiable or falsifiable
A researcher must generate a realistic and
testable hypothesis
Hypothesis
Experiments to be done are built around
the hypothesis
The results of the experiments either
verify or falsify the hypothesis
Types
of hypo-
thesis
Null hypothesisː Meant
to be nullified
Alternative hypothesisː
Meant to be verified
Examples
of hypo-
thesis
Null hypothesisː Drug X does
not decrease serum cholesterol
Alternative hypothesisː Drug X
decreases serum cholesterol
Testing of hypothesis
Design
experiment
Collect data
Examine the
data statistically
Decide the type of subjects
Decide the sample size
Sample size should be large enough
to permit valid statistical analysis
Have an adequately sized group for
comparison
Designing experiments
Choose the experiments (e.g. measure-
ment of serum cholesterol)
Decide upon the instruments, reagents,
etc required
Take ethical clearance before starting
animal or human experiments
Perform
experiments
Collect data
Organize
data
Evaluate
data
Data analysis requires statistical tools
Use of statistics
Using appropriate statistical tests helps
in correct interpretation of data
Statistical tests ensure that the changes
observed are not chance variations
Originality and novelty are of paramount
importance in research
Originality in research
If the problem you are investigating has
already been solved, you will get no credit
In biomedical research, some
repetitive studies may be
justified because of:
Regional
variations
Racial
variations
Ethnic
variations
Repetition is justified if earlier work has
resulted in a controversy
Repetition is specially justified if you are
using:
Bigger
sample
size
Better
experimental
techniques
Better
study
design
Literature search shows us what has
been done about the problem in the past
A thorough literature search is essential
to ensure originality
Literature search
Literature search will show what is known
and what is not known about the problem
What is known can help us in refining the
research question
What is not known can show the way for
new work
Literature search can also provide
information aboutː
The
methods
to be used
The
sample
size
The
type
of study
Literature to be searched can beː
Primary
literature
Secondary
literature
Tertiary
literature
Papers published
in journals
Primary literature
Give authentic
information
Secondary literature
Review articles and
meta-analyses
Compiled from primary
sources and evaluated
Reliable if taken from
high quality sources
Derives information from
primary/secondary literature
Examples are reference
books
Reliability depends upon the
reputation of the author
Tertiary literature
A number of print journals are now
available online also
In many cases, back issues are also
available
Some of these can be viewed or
downloaded only on payment
Online resources
A number of databases are available
online
These include PubMed, MedlinePlus,
Embase, Web of Science, OVID etc
Most of these databases provide only
the Abstracts of papers
No single database can provide all the
information
A number of databases should be
searched to ensure thoroughness
Literature search will also be useful
while writing thesis or paper
Pertinent references will need to be
cited in the thesis or the paper
Ensure accuracy of the references that
you cite
Accuracy of references
Inaccurate references in a thesis can
annoy the examiner
Alternatively, check from a reliable
database
As far as possible, see the original
paper
Inaccurate references in a paper can
annoy the referee
What next?
You have done all the hard work
You have discovered a new fact
Hypothesis has been verified or falsified
Research question has been answered
Hence, you must share the facts
you have discovered
If you keep your research to yourselfː
You will
get no
credit
Others will
tread the
same path
Society
will not be
benefitted
In either case, you have to first write a
research paper
You can disseminate your research
byː
Presenting it in a
conference
Publishing it in a
journal
Papers sent to journals are reviewed
by independent experts (referees)
They are accepted for publication only
after approval by referee(s)
Publications in reputed journals are
read widely and have a long shelf-life
Such publications add to the prestige
of the author
Research - An Overview.pptx

Research - An Overview.pptx

  • 1.
    Research – AnOverview R.C. Gupta MD (Biochemistry) NIMS, Jaipur
  • 2.
    What is research? Nosingle definition Defined in different ways by different persons
  • 3.
    Some definitions A systematiceffort to gain new knowledge A careful investigation for new facts Discovery of new facts and their interpretation Revision of theories in the light of new facts What is common is the word ‘new’
  • 4.
    Research is ... Discoveryof new facts or knowledge Adding new information to existing knowledge New practical application of knowledge
  • 5.
    Scientific research Unlike other disciplines, scientific research doesn’t depend solelyuponː Mental processes Critical thinking Reasoning Personal opinions
  • 6.
    Scientific research requiresscientific method Scientific method generally has four stepsː Making an observation Forming a hypothesis Testing the hypothesis Reaching a conclusion 1 2 3 4
  • 8.
    Observation – DrugX decreases blood pressure Hypothesis – Drug X can be used to treat hypertension Testing – Give drug X to a group of hyper- tensives and monitor blood pressure Conclusion – Drug X is or is not useful in treating hypertension
  • 9.
    Observation – DrugX decreases blood pressure Hypothesis – Drug X is better than the older drug Y in controlling blood pressure Testing – Give drug X and drug Y to two groups and monitor blood pressure Conclusion – Drug X is or is not better than drug Y in treating high blood pressure Controlled study
  • 10.
    Scientific research generallybegins with a research question and/or a hypothesis A hypothesis is an assumption based on some evidence A research question is an unanswered question
  • 11.
    The researcher teststhe hypothesis to find out whether it is correct To test the hypothesis, the researcher has to use certain methods A methodical study would test the hypo- thesis and answer the research question
  • 12.
    Frame the research question Forma hypothesis Collect data to test the hypothesis Interpret data to answer the question Research methodology
  • 13.
    Question Search literature Form hypothesis Conductexperiments Collect data Interpret data Share knowledge New questions arise
  • 14.
    Merit of theresearch depends uponː Selection of problem The “research topic” is really a “problem” which the researcher tries to solve How important the problem is How useful the solution provided is
  • 15.
  • 16.
    Framing the researchquestion Success of research depends upon how well the research question is framed The question tells us whether the problem is relevant, important and researchable The research question should be specific and precise
  • 17.
    Research question –The PICO format P I C Outcome O Population Intervention Comparison
  • 18.
    Population The studypopulation Intervention Treatment, procedure, test etc Comparison The population for comparison Outcome The effect of the intervention The question should describe P, I, C & O
  • 19.
    Is pain medicationmore effective than aerobic stretching in controlling post- operative pain in adult patients with total hip replacement? A sample research question P – Adults with total hip replacement I – Pain medication C – Aerobic stretching O ̶ Post-operative pain
  • 20.
    Research question –Criteria F – Feasible I – Interesting The research question should meet FINER criteria N – Novel E – Ethical R – Relevant
  • 21.
    A research problem,framed as a question, is the precursor to hypothesis Hypothesis is a paring down of the problem into something verifiable or falsifiable A researcher must generate a realistic and testable hypothesis Hypothesis
  • 22.
    Experiments to bedone are built around the hypothesis The results of the experiments either verify or falsify the hypothesis
  • 23.
    Types of hypo- thesis Null hypothesisːMeant to be nullified Alternative hypothesisː Meant to be verified
  • 24.
    Examples of hypo- thesis Null hypothesisːDrug X does not decrease serum cholesterol Alternative hypothesisː Drug X decreases serum cholesterol
  • 25.
    Testing of hypothesis Design experiment Collectdata Examine the data statistically
  • 26.
    Decide the typeof subjects Decide the sample size Sample size should be large enough to permit valid statistical analysis Have an adequately sized group for comparison Designing experiments
  • 27.
    Choose the experiments(e.g. measure- ment of serum cholesterol) Decide upon the instruments, reagents, etc required Take ethical clearance before starting animal or human experiments
  • 28.
  • 29.
    Data analysis requiresstatistical tools Use of statistics Using appropriate statistical tests helps in correct interpretation of data Statistical tests ensure that the changes observed are not chance variations
  • 30.
    Originality and noveltyare of paramount importance in research Originality in research If the problem you are investigating has already been solved, you will get no credit
  • 32.
    In biomedical research,some repetitive studies may be justified because of: Regional variations Racial variations Ethnic variations
  • 33.
    Repetition is justifiedif earlier work has resulted in a controversy Repetition is specially justified if you are using: Bigger sample size Better experimental techniques Better study design
  • 34.
    Literature search showsus what has been done about the problem in the past A thorough literature search is essential to ensure originality Literature search
  • 35.
    Literature search willshow what is known and what is not known about the problem What is known can help us in refining the research question What is not known can show the way for new work
  • 36.
    Literature search canalso provide information aboutː The methods to be used The sample size The type of study
  • 37.
    Literature to besearched can beː Primary literature Secondary literature Tertiary literature
  • 38.
    Papers published in journals Primaryliterature Give authentic information
  • 39.
    Secondary literature Review articlesand meta-analyses Compiled from primary sources and evaluated Reliable if taken from high quality sources
  • 40.
    Derives information from primary/secondaryliterature Examples are reference books Reliability depends upon the reputation of the author Tertiary literature
  • 41.
    A number ofprint journals are now available online also In many cases, back issues are also available Some of these can be viewed or downloaded only on payment Online resources
  • 42.
    A number ofdatabases are available online These include PubMed, MedlinePlus, Embase, Web of Science, OVID etc Most of these databases provide only the Abstracts of papers
  • 43.
    No single databasecan provide all the information A number of databases should be searched to ensure thoroughness
  • 44.
    Literature search willalso be useful while writing thesis or paper Pertinent references will need to be cited in the thesis or the paper Ensure accuracy of the references that you cite
  • 45.
    Accuracy of references Inaccuratereferences in a thesis can annoy the examiner Alternatively, check from a reliable database As far as possible, see the original paper Inaccurate references in a paper can annoy the referee
  • 46.
    What next? You havedone all the hard work You have discovered a new fact Hypothesis has been verified or falsified Research question has been answered
  • 47.
    Hence, you mustshare the facts you have discovered If you keep your research to yourselfː You will get no credit Others will tread the same path Society will not be benefitted
  • 48.
    In either case,you have to first write a research paper You can disseminate your research byː Presenting it in a conference Publishing it in a journal
  • 49.
    Papers sent tojournals are reviewed by independent experts (referees) They are accepted for publication only after approval by referee(s) Publications in reputed journals are read widely and have a long shelf-life Such publications add to the prestige of the author