safety and biosecurity (lab 10).Procedures for Handling Hazardous Spills

Safety and biosecurity
Laboratory Lecture 10:- Procedures for Handling Hazardous
Spills
Dr. Raghda Saad Mohammed

b- Spill Clean Up of Biological Agents
It is important to be aware that there may be potential risks in handling and
processing biological materials (especially those of human),
microorganisms such as bacteria and viruses (e.g. Hepatitis A, B and C
virus, HIV), recombinant DNA, tissue culture and oncogenic viral systems
and other human pathogens that are blood-borne, etc. All spills involving
biological material and agents should therefore be treated as potentially
infectious.

b- Spill Clean Up of BiologicalAgents
As part of the biosafety program, Laboratory has a spill
response kit with appropriate personal protective clothing and
clean up materials located in the laboratory and is available
for use at all times. Spill kits should include absorbent
material appropriate to contain the spill and reduce aerosols.
All biological spills must be attended to immediately. The
approach that take will depend on the risk group of the
biological material, the volume and location of the spill.

Biological Spill Kit
A basic biological spill kit should include:
Personal Protective Equipment
Materials:- Appropriate disinfectant , bottle for making
dilutions of disinfectant , paper towels or other suitable
absorbent materials , forceps or tongs , sharps container for
broken glass or needle, dust pan with broom , yellow biohazard
trash bags , biohazard spill alert notice , biological spill
response procedure and emergency contact numbers.

1-GeneralSpillsClean-upProcedure(outsideofbiologicalsafetycabinet)
 Small spill of Risk group 1 or 2 material (< 100mls)
1. Alert people in immediate area of spill.
2. At a minimum, wear disposable gloves and face protection.
3. Cover spill with paper towel or other absorbent material.
4. Carefully pour a freshly prepared 1:10 dilution of household bleach (or other
effective disinfectant) around the edges of the spill and then into the spill. Avoid
splashing.
5. Allow a 20-minute contact period for bleach (or as indicated as effective time for
different disinfectant). If broken glass is present, use forceps to remove and place
glass in sharps collection container.
6. Use paper towels to wipe up the spill, working from the outer edges into the
center.

Spill Involving Concentrated Microorganisms Required BSL 3 Containment
(e.g. Mycobacterium tuberculosis, (TB) cultures)
Attend to injured or contaminated persons and remove them from exposure.
Alert people in the area to evacuate. Close doors to affected area; do not
enter area for at least one hour. Have a person knowledgeable of the incident
and area assist in proper cleanup. Wearing gowns, gloves, respirator, and
shoe covers, clean up spills as indicated for biohazard spill clean-up.

 Large spill of Risk group 1 material (> 100mls)
1. Get help if required.
2. Clean up procedure is the same as for small risk group 1 spills only on a
larger scale.
3. Notify the lab manager or supervisor that there has been a spill.
 Large spill of Risk group 2 material (> 100mls)
1. Get Help.
2. Contact the lab manager immediately.
3. Keep people out of the area
4. If the spill is potentially infectious the area must be vacated for 30
minutes to allow aerosols to settle before the clean- up procedure can
commence.
5. Follow the instructions inside the Biohazard Spill kit.
6. Remove and decontaminate any PPE (including lab coats) according to
laboratory protocols.
7. Report the incident.

2-SpillinaBiologicalSafetyCabinet
1. Ensure that the cabinet is on and continues to operate during the clean-up
procedure
2. Put on PPE
3. Remove any sharp objects with forceps
4. Cover the spill with absorbent material and dispose of in the biological
waste bin
5. All surfaces must be decontaminated. Cover the affected area with
suitable disinfectant (e.g. 1:10 dilute of sodium hypochorite) and leave for
the appropriate contact time (e.g. 30 minutes).

2-SpillinaBiologicalSafetyCabinet
6. Ensure that the surfaces below the work area are also treated.
7.After decontamination thoroughly rinse the surface to remove any
remaining bleach because it can corrode stainless steel.
8.Items in the BSC at the time of the spill must be thoroughly cleaned with
70% ethanol prior to removal from the BSC and/or bagged for removal and
autoclaved.
9.Wipe the inside of the cabinet with 70% ethanol and allow BSC to run for
10 minutes prior to resuming work.
10. Remove and decontaminate any PPE (including lab coats) according to
laboratory protocols.
11. Report incident.

A biological spill in a centrifuge has the potential for creating aerosols. As soon as the
operator becomes aware of a spill immediate action is required.
1. Turn off centrifuge. Do not open the centrifuge for 30 minutes to allow the aerosols to
disperse and settle; place a note warning other not to open it.
2. Allow 30 minutes settling time before cleaning up procedures commence.
3. Put on PPE. Remove all debris.
4. Place contaminated equipment in leak proof bag and if possible, transfer to Biological
Safety cabinet for disinfection
5. Disinfect (1:10 dilute of sodium hypochorite) the interior of the centrifuge and the head
(or cups).
6. All debris must be collected, bagged, autoclaved, and disposed of appropriately
7, Remove and decontaminate any PPE (including lab coats) according to laboratory
protocols.
8. Report spill.
3- Spill in a centrifuge

4- Biological Spill on Body
1. Remove contaminated clothing.
2. Vigorously wash exposed area with soap and water for 1
minute.
3. Obtain medical attention, if necessary.
4. Report incident

5- Hazardous Material Splashed in Eye
1. Immediately rinse eyeball and inner surface of eyelid with water
continuously for 15 minutes.
2. Forcibly hold eye open to ensure effective wash behind eyelids.
3. Obtain medical attention.
4. Report incident.
6- Minor Cuts and Puncture Wounds
1. Vigorously wash injury with soap and water for several minutes.
2. Obtain medical attention.
3. Report incident.

Thanks for
your
attention
9/17/2022
14

safety and biosecurity (lab 10).Procedures for Handling Hazardous Spills

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