Stomatal frequency (Plant Development)

Stomatal frequency (Plant Development)

• 1.
  DEMONSTRATE STOMATAL FREQUENCY OFSTOMATAL INDEX BY USING EPIDERMAL SURFACE OF LEAF.
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  MATERIALS REQUIRED
• 4.
  Stomatal index It thepercentage which the number of stomata form to the total number of epidermal cells, each stoma being counted as one cell. Stomatal index can be calculated by using the following equation: Stomatal Index = S x 100/E+S S= Number of stomata per unit area. Stomatal number Stomatal number is defined as the average number of stomata per sq mm of epidermis of the leaf. Stomatal frequency can be defined as the number of stomata present per unit area of a leaf.
• 5.
  Procedur e Take a dorsiventralleaf and with the help of forceps carefully peel off epidermal layer from both sides. Then take a clean glass slides and mount the peel leaf on it and a drop of water. Observe under microscope. Count the number of stomata on both surface of leaves in area under microscope. With the help of stage micrometer measure diameter of epidermis under microscope.
• 6.
  Stomatal Index =S x 100/E+S S= Number of stomata per unit area. E= Number of epidermal cells per unit area.
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  RESULT On lower surfaceof leaf both stomatal frequency and stomatal index is higher as compare to upper surface UPPER SURFACE LOWER SURFAC E
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  CONCLUSION With the helpof Stomatal index and Stomatal frequency we can calculate stomatal intensity of upper and lower surface of leaf and it plays important role in determining rate of transpiration and gas exchange. When stomatal frequency is high then rate of transpiration is also high and vice-versa.