STA. CRUZ ACADEMY OF LUBAO, INC.

Sta. Cruz, Lubao, Pampanga

Senior High School Department

TEACHING GUIDE
Academic Year 2022-2023

SUBJECT: GENERAL BIOLOGY 2

GRADE LEVEL: 11
SEMESTER/QUARTER: SECOND SEMESTER / THIRD QUARTER
TEACHER: MS. EFRAIM G. MENDOZA. LPT
Topic/Lesson Name Recombinant DNA Technology
A. Genetic Engineering
B. Recombinant DNA
Content Standard The learners demonstrate an understanding of...
1. Mendel’s Laws of
2. Inheritance
3. Sex Linkage
4. Central Dogma of
5. Molecular Biology
6. Recombinant DNA

Performance Standard 1. make a pedigree analysis in the learner’s family using a simple genetic trait
2. make a research paper/case study/poster on genetic diseases

Most Essential Learning 1. Outline the Process Involved in Genetic Engineering

Competencies 2. Discuss the application of recombinant DNA.

Specific Learning Outcomes 1. Outline the Process Involved in Genetic Engineering

 2. Discuss the application of recombinant DNA.

Time Allotment 720 minutes

I. Introduction

Prayer
Checking of Attendance
Brief introduction of the lesson

Biotechnology includes a wide range of processes. Most people will think biotechnology is synonymous to gene cloning and associate it with creation of the first cloned
mammals. Biotecnology also includes century-old culinary processes and technique that made use of living organisms to creat a product including in the long list is the beer
brewing that uses yeast to ferment malted barley sugar to produce alcohol. The production of penincillin from species of molds used for bacteria infection and gene therapy and
deseases with invoct inserting genes in cells to replace a messing or defection gene. Biotechnology can also be helpful in environmental remediation and food production.A
specialized area of biotechnology is called DNA Technology. This focuses on the ability to analyse, manipulate and cut and paste DNA pieces.

II. Motivation
Activity 1: Q and A

If you’re going to modify an organism, what organism it is and what modifications are you going to do?
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Do you agree on the use of GMOs?

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III. Instruction
 Discussion about the Recombinant DNA Technology

Genetic Engineering - Is a process of making changes on the genetic code of an organism.

Different Techniques Used in Genetic Engineering

1. Identify an organism that contains desirable gene.

2. Extract the entire DNA from the organism.
3. Remove this gene from the rest of DNA.
4. Insert the new gene to an existing organism’s DNA.

Deoxyribonucleic Acid - The DNA is a double-helix molecule with 2 complimentary strands similar to a spiral ladder
with the backbones made up of deoxyribose sugar and phosphates. The steps of the ladder are composed of nitrogenous
bases.

DNA Recombination - -is a process of modifying the genes of organisms for practical purposes.

Brief History of Recombinant DNA Technology

● The first production of recombinant DNA molecules using restriction enzymes, occurred in the early 1970’s. Recombinant DNA technology from different species and
subsequently inserting the hybrid DNA into the host cell often a bacterium.
● The first licensed drug generated using recombinant DNA technology was human insulin.

Technologies And Tools Used In Recombinant DNA

Gel Electrophoresis - is a method used to separate DNA fragments based in their size.
DNA Sequencing - this is a method used to provide the identity and order of nucleotides in a DNA strand.
Polymerase Chain Reaction (PCR) - is a method widely used to rapidly make millions to billions of copies of specific DNA sample
allowing scientists to take a very small sample of DNA and amplify it to a large enough amount to study in detail.

Importance Of DNA Technology

Gene Analysis
The development of the polymerase chain reaction allowed scientists to make numerous copies f DNA segment. When there are
numerous copies of the same gene, scientists can design several researches on the gene to be able to better understand its
functions in the organism.

Gene Therapy - is a form of treatment used to cure condition that arises from the defective or absent gene.

Applications Of Recombinant DNA

Agriculture - Transgenic plants or plants that contains genes from other organisms are now an important part in the field of
agriculture. By using recombinant DNA technology can now be grown with genes responsible for producing natural insecticides.

Medicine
An important contribution of recombinant DNA technology in the field of medicine is the use of bacteria to create substances
that our body needs, whether to maintain good health or to treat a disease.

Food Industry
Recombinant technology has also improved the food industry.

IV. Practice
Activity 2: Quiz

V. Enrichment/Assignment

Activity 3
 Read genetic engineering and DNA recombination page 96, and answer the questions below:

1. What is the goal of genetic engineering?

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2. How is the procedure in genetic engineering?

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3. What is DNA recombination?

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4. How is it done?

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VI. Evaluation

For the assessment of this lesson, you are tasked to draw the structure of a DNA molecule. Give the definition and function of a DNA. Do this on a long bond paper.

Rubric
Criteria Descriptions Score
4 3 2 1
Accuracy of Content The facts, information and The facts, information and The facts, information and The facts, information and
explanation are clearly, explanation are clearly, explanation are clearly, explanation are clearly,
concisely, and logically logically presented with presented with supporting presented with no supporting
presented with supporting supporting information information details
information
Presentation of the Presentation shows consider Presentation shows Presentation shows attempt Presentation shows very
Output ability originality, and originality, and of originality and little attempt of original
inventiveness. It is presented inventiveness. It is presented inventiveness. thought.
 in a unique and interesting in an interesting way.
way.
Creativity The work is very creatively The work is creatively done The work is somewhat The work is not creatively
done and very appealing. It and appealing. It is creatively done and not so done and not appealing.
is appropriate to the topic. appropriate to the topic. appealing.
Total Score

VII. Reference/s

Book: Diwa General Biology 2 Glen S Mirabete– page 96 - 110

Phoenix General Biology 2 Anna Cherylle Morales Ramos page 209
General Biology 2 Luisiyo t. Evangelista page - 190