GLUCOSE
GOD-PAP Method
Enzymatic, Colorimetric Test
PRODUCT CODE SPECIMEN SYMBOL ON LABELS
CS008 Serum or plasma, free of hemolysis
Glucose is stable for 24 hours if serum or plasma is at 2-8° C.
INTENDED USE
This reagent is intended for in vitro quantitative determination of PRECAUTION
Glucose in serum & plasma. To avoid contamination, use clean laboratory wares.
Avoid direct exposure of reagent to light.
CLINICAL SIGNIFICANCE
Glucose is the major carbohydrate present in the peripheral ASSAY
blood. The oxidation of glucose is the major source of cellular Wavelength : 546nm, 500 nm
energy in the body. Glucose determinations are run primarily Cuvette : 1 cm light path
to aid in the diagnosis and treatment of diabetes mellitus. Temperature : 20-25°C or 37°C
Elevated levels glucose levels may be associated with Measurement : Against reagent blank
pancreatitis, pituitary or thyroid dysfunction, renal failure and
liver disease, whereas low glucose levels may be associated PROCEDURE
with insulinoma, hypopituitarism, neoplasms, or insulin Pipette into Blank Standard Sample BIBILOGRAPHY
induced hypoglycemia. cuvettes 1- Trinder, P. determination of Blood Glucose using 4-
Glucose reagent 1000 µL 1000 µL 1000 µL Aminophenazone; J Clin. Path 22 246 1969
PRINCIPLE Standard -- 10 µL -- 2- Teuscher, A, and richterich, P. Schweiz and wochensohr 101
The enzymatic reaction sequence employed in the assay of glucose is Sample -- -- 10 µL 342, 390, 1971
as follows, Mix and incubate for 10 minutes at 20-25°C or 5 minutes at 37°C 3- Dingeon, B.; Ann.Bio.Clin 33,3 (1975)
Glucose Oxidase Measure the absorbance of the sample (As) and standard (Astd)
β-D-Glucose+ O2 + H2O -----------------------> D-Gluconic acid + against the reagent blank.
H2O2
CALCULATION
Peroxidase
∆A sample
H2O2+phenol + 4-Aminoantipyrine -------------------> Quinonimine
Glucose Conc. (mg/dL) = X 100 (Std.conc.)
+ 4H2O
∆A standard
The oxidation of glucose is catalyzed by glucose oxidase (GOD),
To convert mg/dL to mmol divide by 18
the resultant hydrogen peroxide (H2 O2) is oxidatively coupled with
4–Aminophenazone and Phenol in the presence of Peroxidase (POD)
Linearity
to yield a red Quinonimine dye, the concentration of which at 546nm
This reagent is linear up to 400 mg/dL,
is proportional to the concentration of glucose.
If the concentration is greater than linearity (400 mg/dL), dilute the
sample with normal saline and repeat the assay. Multiply the result
REAGENT COMPOSITION with dilution factor.
GLUCOSE (Liquid) Reagent
Phosphate buffer, (pH 7.5) 0.1 mol/L NORMAL RANGE
Phenol 10 mmol/L It is recommended that each laboratory establish its own reference
4-Aminoantipyrine 0.3 mmol/L values. The following value may be used as guide line.
Glucose oxidase 10000 U/L Serum / Plasma: 75 - 115 mg/dL (4.2-6.4 mmol/L)
Peroxidase 700 U/L
GLUCOSE STANDARD QUALITY CONTROL
Glucose standard concentration 100 mg/dL All control sera with Glucose value estimated by this method can be
used.
REAGENT PREPARATION
NOTES
Reagent and standard are ready for use.
1- Physiological concentration of uric acid, ascorbic acid,
glutathione, anticoagulants, bilirubin and Creatinine do not
REAGENT STORAGE AND STABILITY
influence the technic.
- The reagent and standard should be stored at 2 - 8°C, the reagent 2- The reagent contains sodium azide as preservative. Do not
stable until the expiration date indicated on the package label. swallow and avoid contact the skin and mucous membrane.
Bio Research For Medical Diagnostics
MDSS GmbH
Muslim Al Attar Street,P.O.Box:1235, Doc.No.: IFU-CH-016
Schiffgraben 41
Amman-11953,Jordan Rev.: 07
30175 Hannover, Germany
Tel:+962 64892525, Fax: +962 64892526, Page 1 of 1
www.bioresearch.com.jo
