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Knowing The Microscope

This document outlines the educational objectives for understanding microscopes in biology, including their use, parts, and magnification rules. It describes the Light Microscope, its components such as the eyepiece, objective lenses, and adjustment knobs, along with their functions. Additionally, it provides a table of magnification levels for different objective lenses.

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9 views3 pages

Knowing The Microscope

This document outlines the educational objectives for understanding microscopes in biology, including their use, parts, and magnification rules. It describes the Light Microscope, its components such as the eyepiece, objective lenses, and adjustment knobs, along with their functions. Additionally, it provides a table of magnification levels for different objective lenses.

Uploaded by

enriquezkirkerrol350
Copyright
© © All Rights Reserved
We take content rights seriously. If you suspect this is your content, claim it here.
Available Formats
Download as PDF, TXT or read online on Scribd

Name: __________________________________ Date: ____________

Grade & Section: _______________________

KNOWING THE MICROSCOPE

Learning Objectives:

At the of this activity, students will be able to:

a. Determine the use of microscope in biology


b. Identify its parts and function
c. Apply rules of magnification in viewing slide samples.

A. The Light Microscope

Generally, a microscope is an optical instrument used to magnify and observe small objects that
are not visible to the naked eye. One of its types is the Light Microscope. Light Microscope uses
visible light and a system of lenses to magnify images of small samples. It's commonly used in
biology for observing cells, bacteria, and other microorganisms.

B. Parts and Function

1. Head: It is also referred to as the


body tube. The head of the
microscope contains optical
components.
2. Eyepiece: This area is used to
view objects through the
microscope. It is located at the tip
of the microscope.
3. Nose piece: It retains objective
lenses. Because it is mobile, the
objective lenses can rotate
according to the lens’s
magnification.
4. Objective lenses: These are the primary lenses employed for specimen visualization. One
microscope has one to four objective lenses: 1. Scanner (red) 2. Low Power (yellow) 3. High
Power (green/blue) 4. Oil Immersion (white). some of which are forward-facing and others
rear-facing. The magnification power of each lens varies.

5. Base: It serves as a support for microscopes.


6. Arm: It joins the base to the head and the eyepiece tube to the base.
7. Adjustment knobs: They are utilized to focus the microscope. There are two different kinds
of adjustment knobs: coarse adjustment knobs and fine adjustment knobs.
8. Stage: The specimen is displayed here. This comes along with stage clips.
9. Aperture: A hole in the microscope stage via which transmitted light from the source enters
the stage.
10. Microscopic illuminator: It is located at the microscope’s base, providing its light source. It
is utilized in place of a mirror.
11. Condenser: The lenses used to gather and concentrate light from the illuminator into the
sample. They are located near the microscope’s diaphragm under the stage.
12. Diaphragm: It is also called the iris. It can be found under the microscope’s stage, and its
primary function is to regulate how much light gets to the specimen.

C. Rules of Magnification

Scanning Low Power Oil Immersion


High Power
Objective Objective Objective
Objective Lens
Lens Lens Lens
Magnification 4X 10X 40X 100X
Total Magnification
40X 100X 400X 1000X
with 10x Eyepiece Lens

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